Effect of white African mineral hair dye on the activities of phosphatases and malondialdehyde level in selected tissues of albino rats

• Published in: Toxicological and environmental chemistry Vol. 87; no. 3; pp. 399 - 406
• Main Authors: Adebayo, G. B, Sunmonu, T. O, Adekola, F. A, Olatunji, G. A
• Format: Journal Article
• Language: English
• Published: Taylor & Francis Group 01-07-2005
• Subjects: cancer; Dye; hydrogen peroxide; malondialdehyde; phosphatases; skin; tumour
• ISSN: 0277-2248; 1029-0486
• DOI: 10.1080/02772240500165539

The effect of white African mineral dye Yombofita (YF) on the activities of alkaline phosphatase (ALP), acid phosphatase (ACP) and malondialdehyde (MDA) levels in the skin, liver, kidney and serum of albino rats was investigated. The chemical analysis of the dye was first carried out using solubility test, pH determination and X-ray fluorescence (XRF) elemental analysis. Six different concentrations (0.05, 0.15, 0.25, 0.5, 0.75 and 1.00%) of the dye were prepared using hydrogen peroxide (30 volume) as solvent. A total of 80 albino rats (Rattus norvegicus) were used for the study. The rats were divided into 8 groups of 10 each and were maintained on commercial feed for the period of the experiment i.e. 30 days. In group 1, the control group, the animals were applied distilled water on their heads, whereas in group 2 the vehicle i.e. hydrogen peroxide was applied. In groups 3 to 8 various concentrations of YF (white) dye ranging from 0.05, 0.15, 0.25, 0.5, 0.75 to 1.00% was applied respectively. At the end of the experiment, blood samples were collected and portions of the selected tissues were excised for the determination of ALP and ACP activities. The MDA level was also determined in the skin of experimental animals. The results revealed a significant decrease (p < 0.05) with increasing concentration of the dye in the activities of ALP and ACP in the kidney and skin but not in the liver while the level of MDA in the skin increased significantly (p < 0.05) with increasing concentration of the dye.