Ultrastructure and mitochondrial vesiculation associated with closteroviruslike particles in leafroll-diseased grapevines

• Published in: Phytopathology Vol. 79; no. 3; pp. 357 - 360
• Main Authors: Kim, K.S, Gonsalves, D, Teliz, D, Lee, K.W
• Format: Journal Article
• Language: English
• Published: St. Paul, MN American Phytopathological Society 01-03-1989
• Subjects: Biological and medical sciences; ELISA; ESTRUCTURA CELULAR; FLOEMA; Fundamental and applied biological sciences. Psychology; IMMUNOLOGIE; INMUNOLOGIA; MICROSCOPIA; MICROSCOPIE; MITOCHONDRIE; MITOCONDRIA; PARENCHYME; PARENQUIMA; Pathology. Damages, economic importance; PHLOEME; Phytopathology. Animal pests. Plant and forest protection; Plant viruses and viroids; STRUCTURE CELLULAIRE; TEST ELISA; ULTRAESTRUCTURA; ULTRASTRUCTURE; VIRUS DE LAS PLANTAS; VIRUS DES VEGETAUX; VITIS; Virus; Fruit crop; Ultrastructure; Cytology; Plant leaf; Phytopathogen; Method; Electron microscopy; ELISA assay
• ISSN: 0031-949X; 1943-7684
• DOI: 10.1094/Phyto-79-357

Ultrastructural studies of Riesling and Chardonnay grapevines with typical leafroll-disease symptoms occurring in northwest Arkansas revealed flexous, rod-shaped viruslike particles approximately 12 nm in diameter and of undetermined length occurring consistently in phloem cells. In phloem parenchyma cells, the particles were associated with clusters of membranous vesicles, 50-100 nm in diameter, containing fine fibrils characteristic of closterovirus infections. Unlike other known closteroviruses, however, the fibril-containing vesicles originated from modified mitochondria. Direct enzyme-linked immunosorbent assays indicated that the virus was serologically related to a New York isolate (NY-1) of a closterovirus associated with grapevine leafroll disease