Regulation of the strain Citrobacter freundii Ml-31.1/1 interaction with iron compounds

• Published in: Biotechnologia acta Vol. 8; no. 5; pp. 103 - 111
• Main Authors: Govorukha, V M, Tashyrev, O B
• Format: Journal Article
• Language: English
• Published: Kyiv Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine 01-01-2015; National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry
• Subjects: Citrobacter freundii; environment protection biotechnologies; immobilization; iron biogeochemical cycles; iron compounds; mobilization; regulation of microbial interaction with iron; thermodynamic prognosis
• ISSN: 2410-7751; 1995-5537; 2410-776X
• DOI: 10.15407/biotech8.05.103

The aim of the work was to show the possibility for the strain Citrobacter freundii Ml-31.1/1 on the base of its metabolism regulation to carry out two opposite processes: mobilization of iron compounds and their immobilization. To do this investigation thermodynamic calculations were used. It was also used potentiometric measuring of pH and Eh values, colorimetric determination of microbial biomass increase and changes of iron compounds concentration and gas chromatography. The possibility of theoretically grounded regulation of iron transformation pathways was experimentally confirmed. Taking as an example the culture C. freundii Ml-31.1/1, we have shown its ability to provide both immobilization and mobilization of iron compounds under specific conditions for microbial growth in nutrient medium. Obtained regularities are the basis to estimate the role of microorganisms in iron biogeochemical cycles. These data could be used as a base for the development of effective biotechnological approaches such as iron extraction from depleted deposits and water purification from iron compounds.