HPLC-TOF-MS analysis of metabolites of Diffusa effective extracts in rats

• Published in: Zhongguo zhongyao zazhi Vol. 36; no. 10; p. 1301
• Main Authors: Yan, Lizhi, Wang, Yingfeng, Yu, Qirong
• Format: Journal Article
• Language: Chinese
• Published: China 15-05-2011
• Subjects: Animals; Chromatography, High Pressure Liquid - methods; Drugs, Chinese Herbal - administration & dosage; Drugs, Chinese Herbal - analysis; Drugs, Chinese Herbal - metabolism; Hedyotis - chemistry; Male; Mass Spectrometry - methods; Rats; Rats, Sprague-Dawley
• ISSN: 1001-5302
• DOI: 10.4268/cjcmm20111011

To analyze p-coumaril acid, trans-6-O-p-coumaroyl scandoside methyl ester and its metabolites in rat plasma after intragastric administration of exocarpium Diffusa effective extracts.
Rat blood samples were collected 1.0 h after oral administration of 0.4 g x kg(-1) exocarpium Diffusa effective extracts, and then were analyzed by using HPLC-Q-TOF-MS.
In rat plasma, only one metabolite was detected. The structure was identified by reference substance to be p-coumaril acid.
The methyl glucoside metabolite in rat from the main component parts of trans-6-O-p-coumaroyl scandoside methyl ester droppings of vines in Diffusa effective extracts is p-coumaril acid. This experiment provides a theortical basis for studying chemical compositions and pharmacodynamic action of Diffusa.