Biocontrol prospective of Bacillus siamensis-AMU03 against Soil-borne fungal pathogens of potato tubers

• Published in: Indian phytopathology Vol. 75; no. 1; pp. 179 - 189
• Main Authors: Hussain, Touseef, Khan, Abrar Ahmad
• Format: Journal Article
• Language: English
• Published: New Delhi Springer India 01-03-2022
• Subjects: Biomedical and Life Sciences; Life Sciences; Plant Pathology; Research Article; Biosurfactant; Antifungal; Biocontrol
• ISSN: 0367-973X; 2248-9800
• DOI: 10.1007/s42360-021-00447-8

Twelve bacterial strains (GJ01 to GJ12), isolated from an oil-contaminated soil, were characterized through different biochemical, morphological, and molecular technique and analyzed by different screening methods to choose the best biosurfactant producing strain. Only GJ-01 strain exhibited significant biosurfactant activity, while the other strains exhibited partial activity. Molecular identification through 16S  rRNA technique identified GJ-01 as a
Bacillus siamensis-
AMU03
. The effect of the cultural filtrate tested under in vitro study against
Rhizoctonia solani
and
Fusarium oxysporum
, with the highest reduction of mycelial growth in dual culture method (89.40% and 90.20% respectively). Both in food poisoning method as well as pathogenicity test, the bioagent was able to inhibit the growth of both the pathogens successfully at 3% concentration, and that was mainly due to volatile metabolites such as surfactin, fengycin and iturin, which were identified and analysed through Liquid chromatography-mass spectrometry. These findings indicated that
AMU03
strain had a promising eco-friendly and bio-safe approach to cope against soil-borne and post-harvest pathogens of seed tubers.