In vitro androgenesis and segregation distortion in Brassica napus L. : spontaneous versus colchicine doubled lines

• Published in: Plant cell reports Vol. 16; no. 7; pp. 464 - 468
• Main Authors: FOISSET, N, DELOURME, R, LUCAS, M.-O, RENARD, M
• Format: Journal Article
• Language: English
• Published: Berlin Springer 01-04-1997; Springer Verlag
• Subjects: Anther and ovule culture, utilization for haploid production; Biological and medical sciences; Biotechnology; Classical genetics, quantitative genetics, hybrids; Eukaryotic cell cultures; Fundamental and applied biological sciences. Psychology; Genetics; Genetics of eukaryotes. Biological and molecular evolution; Life Sciences; Methods. Procedures. Technologies; Plant cells and fungal cells; Plants genetics; Pteridophyta, spermatophyta; Vegetals; Vegetals; Genetic marker; Tissue culture; Oil plant(vegetal); Androgenesis; Segregation analysis; Haploidy; Cruciferae; Dicotyledones; Gametophyte; Angiospermae; Brassica napus; Spermatophyta; Pretreatment; COLZA
• ISSN: 0721-7714; 1432-203X
• DOI: 10.1007/s002990050261

A total of 750 plantlets were regenerated from 1,400 embryos produced through microspore cultures from one F sub(1) plant of the cross 'Darmor-bzh' x 'Yudal'. Fifty-three percent of the regenerants were evaluated by flow cytometric analysis, which revealed that 31% were spontaneous diploid (SD), 63% were still haploid and the remaining 6% plants had other ploidy levels. Available segregation data (266 markers) produced on this androgenic progeny were used to study the interference between segregation distortion and the mode of chromosome doubling of androgenic lines. On the basis of the present results it is not possible to conclude that distortions are peculiar to one type of regenerated plant; only a difference in the intensity of the bias might be assumed.