19F NMR measurements of NO production in hypertensive ISIAH and OXYS rats

• Published in: Biochemical and biophysical research communications Vol. 330; no. 2; pp. 367 - 370
• Main Authors: Bobko, Andrey A., Sergeeva, Svetlana V., Bagryanskaya, Elena G., Markel, Arkadii L., Khramtsov, Valery V., Reznikov, Vladimir A., Kolosova, Nataljya G.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 06-05-2005
• Subjects: 19F NMR spin-trapping; Animals; Fluorinated probes; Fluorine; Free radicals; Hypertension - metabolism; Hypertensive rats; Magnetic Resonance Spectroscopy - methods; Nitric oxide; Nitric Oxide - biosynthesis; Nitronyl nitroxides; NO scavenging; Rats; Free radicals; Nitric oxide; Nitronyl nitroxides; NO scavenging; Hypertensive rats; 19F NMR spin-trapping; Fluorinated probes
• ISSN: 0006-291X; 1090-2104
• DOI: 10.1016/j.bbrc.2005.02.166

Recently we demonstrated the principal possibility of application of
19F NMR spin-trapping technique for in vivo
NO detection [Free Radic. Biol. Med. 36 (2004) 248]. In the present study, we employed this method to elucidate the significance of
NO availability in animal models of hypertension. In vivo
NO-induced conversion of the hydroxylamine of the fluorinated nitronyl nitroxide (HNN) to the hydroxylamine of the iminonitroxide (HIN) in hypertensive ISIAH and OXYS rat strains and normotensive Wistar rat strain was measured. Significantly lower HIN/HNN ratios were measured in the blood of the hypertensive rats. The NMR data were found to positively correlate with the levels of nitrite/nitrate evaluated by Griess method and negatively correlate with the blood pressure. In comparison with other traditionally used methods
19F NMR spectroscopy allows in vivo evaluation of
NO production and provides the basis for in vivo
NO imaging.