Energy and Pro-/Antioxidant Metabolism of Heracleum sosnowskyi Manden. Buds during the Winter Dormancy

• Published in: Russian journal of plant physiology Vol. 71; no. 4
• Main Authors: Maslova, S. P., Shelyakin, M. A., Silina, E. V., Malyshev, R. V., Dalke, I. V.
• Format: Journal Article
• Language: English
• Published: Moscow Pleiades Publishing 01-08-2024; Springer Nature B.V
• Subjects: Antioxidants; Autumn; Biomedical and Life Sciences; Buds; Dormancy; Energy balance; Energy metabolism; Energy storage; Heracleum sosnowskyi; Hydrogen peroxide; Insolation; Life Sciences; Metabolism; Morphogenesis; Overwintering; Oxidants; Oxidizing agents; Plant Physiology; Plant Sciences; Research Papers; Respiration; Snow cover; Soil temperature; Spring; Spring (season); Thiobarbituric acid; Winter; pro-oxidants; energy balance; antioxidant enzymes; buds; dormancy; cytochrome and alternative respiratory pathways
• ISSN: 1021-4437; 1608-3407
• DOI: 10.1134/S1021443724605780

Data on changes in energy status, activity of pro-/antioxidant metabolism during overwintering of
Heracleum sosnowskyi
vegetative buds were obtained. The buds of
H. sosnowskyi
are not endodormant and their growth is limited by the decrease of the soil temperature to negative values at the end of November. The optimum temperature for energy storage in autumn was found to be in the range of low positive temperatures (2–5°C). The autumn buds were characterized a high capacity for the cytochrome respiratory pathway (CP,
V
cyt
), which accounted for 78% of the total respiration. During autumn morphogenesis and winter dormancy of buds, the levels of pro-oxidants, which are the content of thiobarbituric acid reactive substances (TBARS) and H
2
O
2
content, remained stable. In December, when a stable snow cover and negative soil temperatures were established, the dormant buds showed 2.5 times lower rate of energy storage and activated alternative respiration capacity (AP,
V
alt
), as indicated by a 2 times lower
V
cyt
/
V
alt
ratio. In early spring, compared to winter dormancy, H
2
O
2
levels increased 2-fold and antioxidant enzymes activity increased by 27–78% as insolation increased. Spring buds showed an increase in rate of heat production and a decrease in rate of energy storage, which may be due to spring stress caused by increased insolation. It was concluded that in the tissues of
H. sosnowskyi
buds subjected to exogenous dormancy, an energy balance between dormancy and growth processes is achieved at the level of respiratory capacity and pro-/antioxidant metabolism.