Comparative Analysis of the Formation of γH2AX Foci in Human Mesenchymal Stem Cells Exposed to 3H-Thymidine, Tritium Oxide, and X-Rays Irradiation

• Published in: Bulletin of experimental biology and medicine Vol. 166; no. 1; pp. 178 - 181
• Main Authors: Vorob’eva, N. Yu, Kochetkov, O. A., Pustovalova, M. V., Grekhova, A. K., Blokhina, T. M., Yashkina, E. I., Osipov, A. A., Kabanov, D. I., Surin, P. P., Barchukov, V. G., Osipov, A. N.
• Format: Journal Article
• Language: English
• Published: New York Springer US 01-11-2018; Springer Nature B.V
• Subjects: Biomedical and Life Sciences; Biomedicine; Bone marrow; Cell Biology; Comparative analysis; Deoxyribonucleic acid; DNA; DNA damage; DNA repair; Internal Medicine; Laboratory Medicine; Mesenchymal stem cells; Mesenchyme; Pathology; Stem cells; Thymidine; Translated from Kletochnye Tekhnologii v Biologii i Meditsine (Cell Technologies in Biology and Medicine); Tritium; X-rays; H-thymidine; tritium oxide; DNA double-strand breaks; mesenchymal stem cells
• ISSN: 0007-4888; 1573-8221
• DOI: 10.1007/s10517-018-4309-1

We performed a comparative study of the formation of γН2АХ foci (a marker of DNA doublestrand breaks) in human bone marrow mesenchymal stem cells after 24-h incubation with
3
Н-thimidin and tritium oxide with low specific activities (50-800 MBq/liter). The dependence of the number of γH2AX foci on specific activity of 3H-thymidine was described by a linear equation
y
=2.21+43.45
x
(
R
2
=0.96), where
y
is the number of γH2AX foci per nucleus and
x
is specific activity in 1000 MBq/liter. For tritium oxide, the relationship was described by a linear equation
y
=2.52+6.70
x
(
R
2
=0.97). Thus, the yield of DNA double-strand breaks after exposure to
3
H-thymidine was 6.5-fold higher than after exposure to tritium oxide. Comparison of the effects of tritium oxide and X-ray radiation on the yield of DNA double-strand breaks showed that the relative biological efficiency of tritium oxide in a dose range of 3.78-60.26 mGy was 1.6-fold higher than that of X-ray radiation. Improvement of the methods of analysis of DNA double-strand breaks repair foci is highly promising in the context of creation of highly sensitive biodosimetry technologies for tritium compounds in humans.