Research on the dual mode of JTG-GelMA hydrogel system to promote the regeneration of degenerated intervertebral disc

• Published in: European polymer journal Vol. 220; p. 113475
• Main Authors: Zhang, Yongfeng, Gao, Rong, Xie, Xueyong, Zhang, Jiawei, Liang, Zhuowen, Wei, Zhao, Xu, Feng, Ding, Tan
• Format: Journal Article
• Language: English
• Published: Elsevier Ltd 15-11-2024
• Subjects: extracellular matrix (ECM); GelMA hydrogel; Intervertebral disc degeneration (IVDD); JTG capsule; Matrix stiffness; GelMA hydrogel; extracellular matrix (ECM); Intervertebral disc degeneration (IVDD); Matrix stiffness; JTG capsule
• ISSN: 0014-3057
• DOI: 10.1016/j.eurpolymj.2024.113475

Schematic diagram of the study on the dual mode of JTG-GelMA scaffold to promote the regeneration of degenerated intervertebral disc.
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•Jintiange Capsule was used for the first time in the treatment of intervertebral disc degeneration.•The effects of hydrogel matrix hardness on the phenotype and growth of nucleus pulposus cells were reported.•The cellular and tissue mechanics in the process of intervertebral disc degeneration were discussed.•A rat disc degeneration model was established to simulate the changes of lumbar disc in human daily life.
Diseases such as low back pain and disc protrusion, stemming from intervertebral disc degeneration, have emerged as notable global public health challenges. Researchers have dedicated considerable efforts to delaying the onset of intervertebral disc degeneration through timely intervention, thereby fostering the regeneration of degenerative intervertebral discs. In this investigation, an integration of traditional Chinese medicine theory with contemporary research methodologies was undertaken to devise a composite treatment regimen employing varied concentrations of artificial tiger bone powder (JTG) and GelMA gel. The effect of JTG-GelMA hydrogel on the regeneration of degenerative intervertebral discs was scrutinized via both in vitro and in vivo investigations. The results demonstrated that JTG-GelMA hydrogel promotes the regeneration of degenerative intervertebral discs through dual mechanisms of improving the matrix stiffness of NP cells and inhibiting ECM degradation. Furthermore, a positive correlation between the regenerative efficacy and JTG concentration was observed. This research introduces a novel approach for early intervention in intervertebral disc degeneration and furnishes a theoretical framework for the innovative utilization of traditional medicinal formulations.