Engineering of Bacillus megaterium for improving PHA production from glycerol

There are a few PHA-producer bacteria that can uptake glycerol to produce this biopolymer. Among them, Bacillus megaterium LVN01 has demonstrated to be able to grow up using glycerol as a carbon source. Glycerol dehydrogenase (GD) plays a key role in the synthesis of PHA from glycerol. In this study...

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Bibliographic Details
Published in:	Asia-Pacific journal of molecular biology and biotechnology Vol. 27; no. 3; p. 64
Main Authors:	Cardozo, Javier Ricardo Gómez, Bucheli, Rodrigo Velasco, Sánchez, Carlos del Cerro, Riesco, Isabel de la Mata, Martínez, Amanda Lucía Mora
Format:	Journal Article
Language:	English
Published:	Kuala Lumpur Malaysian Society for Molecular Biology and Biotechnology 2019
Subjects:	Bacillus megaterium Biodegradable materials Biopolymers Carbon sources Glycerol Glycerol dehydrogenase Polyhydroxybutyrate
Online Access:	Get full text
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Summary:	There are a few PHA-producer bacteria that can uptake glycerol to produce this biopolymer. Among them, Bacillus megaterium LVN01 has demonstrated to be able to grow up using glycerol as a carbon source. Glycerol dehydrogenase (GD) plays a key role in the synthesis of PHA from glycerol. In this study, the improvement of glycerol uptake by a recombinant strain of B. megaterium carrying pHT01-bmgd was evaluated in order to enhance PHA production. The biomass and PHA production were evaluated and compared to wild-type. It was determined that the PHA produced by both strains was PHB and the highest improvement in PHB yield was 226% at 30 h.
ISSN:	0128-7451
DOI:	10.35118/apjmbb.2019.027.3.07