Kinetic properties of Na(+),K(+)-АТРase of spermatozoa from fertile and infertile men under effect of calix[4]arene C-107

• Published in: Ukrainian biochemical journal Vol. 91; no. 3; pp. 56 - 64
• Main Authors: Fafula, R. V., Meskalo, O. I., Besedina, A. S., Nakonechnyi, Io. A., Vorobets, D. Z., Vorobets, Z. D.
• Format: Journal Article
• Language: English
• Published: National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry 15-05-2019
• Subjects: calixarene С-107; male infertility; Na(+)-K(+)-ATPase; pathospermia
• ISSN: 2409-4943; 2413-5003
• DOI: 10.15407/ubj91.03.056

The calix[4]arene C-107 (5,17-diamino(2-pyridyl)methylphosphono-11,23-di-tret-butyl-26,28-dihydro­xy-25,27-dipropoxy-calix[4]arene) effects on the kinetic properties of Na+,K+-ATPase in spermatozoa of fertile (normozoospermia) and infertility men (oligozoospermia, and asthenozoospermia) were studied. It was shown that in spermatozoa of healthy men calix[4]arene С-107 inhibited Na+,K+-ATPase activity and decreased the maximum reaction rate of ATP hydrolase reaction without affecting the coefficient of (half-) activation by ATP and Hill coefficient nH. In оligo- and asthenozoospermic samples of spermatozoa almost a 2-fold decrease of cooperativity coefficient nH of ATPase inhibition with calyx[4]aren C-107 was observed. In normozoospermic samples of spermatozoa the KMgCl2 for Na+,K+-ATPase was decreased at calix[4]arene C-107 high concentrations (≥50 nM) in the incubation medium in contrast to oligozoospermic samples of spermatozoa where KMgCl2 was increased only at high calix[4]arene C-107 concentration (100 nM). The increase of the KMgCl2 in the entire range of investigated calix[4]arene concentrations and the decrease of cooperativity coefficient nH of MgCl2 activating effect were detected in asthenozoospermic samples of Na+­,K+-ATPase.