Effect of native plant growth promoting osmotolerant bradyrhizobium strains on drought stress tolerance in retama dasycarpa

• Published in: Applied soil ecology : a section of Agriculture, ecosystems & environment Vol. 203; p. 105662
• Main Authors: Lamrabet, Mouad, Chaddad, Zohra, Bouhnik, Omar, Kaddouri, Kaoutar, Alami, Soufiane, Bennis, Meryeme, Mnasri, Bacem, Abdelmoumen, Hanaa, El Idrissi, Mustapha Missbah
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01-11-2024
• Subjects: Bradyrhizobium; Drought stress; Inoculum; PGP activities; Retama dasycarpa; Rhizobia-legume symbiosis; Drought stress; Rhizobia-legume symbiosis; Retama dasycarpa; Bradyrhizobium; Inoculum; PGP activities
• ISSN: 0929-1393
• DOI: 10.1016/j.apsoil.2024.105662

The application of rhizobia-legume symbioses is a sustainable approach to alleviate water stress and restore damaged areas. In this context, three strains Bradyrhizobium sp. BA2, RDI18 and RDT46 previously isolated from root nodules of Retama dasycarpa grown in the Moroccan High Atlas Mountains, were selected to investigate their prominent drought-tolerance capacity and significant plant growth-promoting (PGP) traits under drought stress. Subsequently, we analyzed the impact of individual or combined inoculations by the three strains on R. dasycarpa responses to three water regimes (40, 70, and 100 field capacity). The three strains tolerate different concentrations of PEG 6000 and possess different PGP activities, including phosphate solubilization, production of siderophore, exopolysaccharides, and auxin, under osmotic stress. The inoculation had a positive impact on plant response under all applied water regimes as it improved shoot and root length biomass, and chlorophyll content. The water stress reduced shoot length and dry weight of all plants. However, the inoculated plants maintained the highest values. The water stress reduced the infectivity of strains BA2 and RDI18, but not strain RDT46, which is not competitive at any water regime. Furthermore, water stress had no effect on the three strains' symbiotic efficiency, whereas it increased considerably the efficiency index of strains BA2 and RDI18. Proline and protein content increased in non-inoculated plants; whereas the inoculation significantly increased the catalase activity in plants under 40 % FC. These results show that the inoculation with appropriate strains such as BA2 and RDI18, enhance plant resilience to drought season.