Lophiosilurus alexandri Functional Studies Related to Mitochondrial Bioenergetics

• Published in: Proceedings of the Zoological Society Vol. 76; no. 2; pp. 190 - 201
• Main Authors: Cavalcante, Sarah K. M., da Silva Santos, João Arthur, Ferreira, Erick José Braga, Mattos, Suzanna Tamy Viana, Nunes, Ábner Magalhães, Saraiva, Karina Lidiane Alcântara, de Figueiredo, Regina Célia Bressan Queiroz, da Silva-Filho, Reginaldo Correia, Fernandes, Mariana Pinheiro, Maia, Maria Bernadete Sousa, Miranda, Edma Carvalho, Leite, Ana Catarina Rezende
• Format: Journal Article Conference Proceeding
• Language: English
• Published: New Delhi Springer India 01-06-2023; Springer Nature B.V
• Subjects: Animal Anatomy; Animal Genetics and Genomics; Antioxidants; Biodiversity; Bioenergetics; Biomedical and Life Sciences; Conservation Biology/Ecology; Electron transport chain; Enzymatic activity; Enzymes; Glutathione peroxidase; Histology; Hydrogen peroxide; Life Sciences; Liver; Lophiosilurus alexandri; Membrane potential; Metabolism; Mitochondria; Morphology; Physical characteristics; Research Article; Respiration; Superoxide dismutase; Transmission electron microscopy; Zoology; Reactive oxygen species; Morphology; Bioenergetic
• ISSN: 0373-5893; 0974-6919
• DOI: 10.1007/s12595-023-00493-2

Lophiosilurus alexandri
(pacamã) is a native fish of the São Francisco River in Brazil. Importantly, its liver bioenergetic metabolism has never been investigated. As the center of metabolism in any aerobic cell is the mitochondria, we sought to investigate some bioenergetic parameters of hepatic mitochondria from pacamã and compare them to those of
Oreochromis niloticus
(tilápia). The activity of each respiratory chain complex, H
2
O
2
and membrane potential were evaluated in isolated liver mitochondria. Some hepatic antioxidant enzymes activity and the mitochondrial structure using transmission electron microscopy were also evaluated. A comparison of tilápia and pacamã revealed that the mitochondrial respiratory control was lower in pacamã, for both complex I (20%) and complex II (28%). The mitochondria from pacamã were also sensitive to Ca
2+
, and produced higher amounts of H
2
O
2
(19%) than were produced in tilápia. Additionally, liver antioxidant enzyme activity levels (catalase and glutathione peroxidase) in pacamã were significantly decreased. However, SOD activity was enhanced. Finally, ultrastructural analysis showed morphological characteristics of stress. In conclusion, liver mitochondria in pacamã seems to have functional respiratory chain complexes, membrane potential Ca
2+
sensitive, produces H
2
O
2
and have an active antioxidant enzyme system, these results are in agreement with the ultrastructural evaluation.