Toxicity parameters of a new 1,2,4-triazole derivative when subcutaneously injected to guinea pigs

• Published in: Regulatory mechanisms in biosystems Vol. 15; no. 1; pp. 166 - 170
• Main Authors: Zazharskyi, V. V., Bigdan, O. A., Parchenko, V. V., Karpenko, Y. V., Zazharska, N. M., Mykhailiuk, Y. O., Kulishenko, O. M., Davydenko, P. O., Kulish, S. M., Gutyj, B. V.
• Format: Journal Article
• Language: English
• Published: Oles Honchar Dnipro National University 27-02-2024
• Subjects: safety; acute toxicity; chronic toxicity; enzymes; biochemical parameters
• ISSN: 2519-8521; 2520-2588
• DOI: 10.15421/022424

The results of the literature analysis indicate a wide spectrum of biological activity of 1,2,4-triazole derivatives. Low toxicity, reactivity, and high biological activity of 1,2,4-triazole derivatives make this class of compounds very attractive. The obtained data determine the relevance of further studies of 1,2,4-triazole derivatives to find new highly effective biologically active substances that can become the basis for new medicines. Our further work aimed to study some toxicity parameters of 3-(3-fluorophenyl)-6-(4-methoxyphenyl)-7H-[1,2,4]-triazolo-[3,4-b][1,3,4] thiadiazine when administered subcutaneously to guinea pigs, which is a necessary condition for further implementation in practice of this compound. In this work, we observed an absence of toxic effects after a single subcutaneous injection to guinea pigs at a dose of 40 mg/kg experimental drug. The results of macro- and microscopic examinations of internal organs 14 days after a single subcutaneous injection of the studied compound at a dose of 20, 40 mg/kg showed the absence of any anatomical and morphological disorders in the tissue structures of guinea pigs. The calculated value indicates a high degree of safety of 3-(3-fluorophenyl)-6-(4-methoxyphenyl)-7H-[1,2,4]-triazolo-[3,4-b][1,3,4] thiadiazine and its prospects for use in veterinary practice as an effective and safe tuberculocidal agent.