Antiviral activity of the Indian medicinal plant extract, Swertia chirata against herpes simplex viruses: A study by in-vitro and molecular approach

• Published in: Indian journal of medical microbiology Vol. 26; no. 4; p. 322
• Main Authors: Gopalkrishna, V, Verma, H, Patil, PR, Kolhapure, RM
• Format: Journal Article
• Language: English
• Published: Chandigarh Elsevier Limited 01-10-2008
• Subjects: Deoxyribonucleic acid; Dilution; DNA; Experiments; Folk medicine; Genes; Herpes viruses; Regression analysis; Viral infections
• ISSN: 0255-0857; 1998-3646
• DOI: 10.4103/0255-0857.43561

Purpose: The antiviral activity of Indian Medicinal plant extract Swertia chirata was tested against Herpes simplex virus (HSV) type-1, using multiple approaches both at cellular and molecular level. Methods: Cytotoxicity, plaque reduction, virus infectivity, antigen expression and polymerase chain reaction (PCR) assays were conducted to test the antiviral activity of the plant extract. Results: Swertia plant crude extract (1gm/mL) at 1:64 dilution inhibited HSV-1, plaque formation at more than 70% level. HSV antigen expression and time kinetics experiments conducted by indirect immunofluorescence (IFA) test, revealed a characteristic pattern of small foci of single fluorescent cells in Swertia extract treated HSV-1 infected cells at 4 hours post infection dose, suggested drug inhibited viral dissemination. Infected cell cultures treated with Swertia extract at various time intervals, tested by PCR, failed to show amplification at 12, 24-72 hours. HSV-1 infected cells treated with Acyclovir (antiviral drug) did not show any amplification by PCR. Conclusions: In this preliminary study, the Indian medicinal plant extract, Swertia chirata showed antiviral properties against Herpes simplex virus type-1.