Characterization and mapping of R Pi-ber , a novel potato late blight resistance gene from Solanum berthaultii

• Published in: Theoretical and applied genetics Vol. 112; no. 4; pp. 674 - 687
• Main Authors: Rauscher, G. M., Smart, C. D., Simko, I., Bonierbale, M., Mayton, H., Greenland, A., Fry, W. E.
• Format: Journal Article
• Language: English
• Published: Heidelberg Springer Nature B.V 01-02-2006
• Subjects: Crop diseases; Genes; Genetics
• ISSN: 0040-5752; 1432-2242
• DOI: 10.1007/s00122-005-0171-4

Phytophthora infestans, the causal agent of late blight, threatens potato production worldwide. An important tool in the management of the disease is the use of resistant varieties. Eleven major resistance genes have been identified and introgressed from Solanum demissum. However, new sources of resistance are continually sought. Here, we report the characterization and refined genetic localization of a resistance gene previously identified as Rber in a backcross progeny of Solanum tuberosum and Solanum berthaultii. In order to further characterize Rber, we developed a set of P. infestans isolates capable of identifying each of the 11 R-genes known to confer resistance to late blight in potato. Our results indicate that Rber is a new resistance gene, different from those recognized in S. demissum, and therefore, it has been named R ^sub Pi-ber^ according to the current system of nomenclature. In order to add new molecular markers around R ^sub Pi-ber^, we used a PCR-based mapping technique, named MASP-map, which located R ^sub Pi-ber^ in a 3.9 cM interval between markers CT240 and TG63 on potato chromosome X. The location of R ^sub Pi-ber^ coincides with an area involved in resistance to different pathogens of potato and tomato.[PUBLICATION ABSTRACT]