Differential effects of ?Advanced glycation endproducts? and ?-amyloid peptide on glucose utilization and ATP levels in the neuronal cell line SH-SY5Y

• Published in: Journal of Neural Transmission Vol. 111; no. 3; pp. 427 - 439
• Main Authors: Kuhla, B., Loske, C., Garcia de Arriba, S., Schinzel, R., Huber, J., M nch, G.
• Format: Journal Article
• Language: English
• Published: Wien Springer Nature B.V 01-03-2004
• ISSN: 0300-9564; 1435-1463
• DOI: 10.1007/s00702-003-0038-2

[beta]-amyloid peptide (A[beta]) and "Advanced glycation endproducts" (AGEs) are components of the senile plaques in Alzheimer's disease patients. It has been proposed that both AGEs and A[beta] exert many of their effects, which include the upregulation of pro-inflammatory cytokines, through RAGE ("receptor for advanced glycation endproducts"). To investigate whether A[beta] and AGEs cause similar or identical effects on cell survival and energy metabolism, we have compared the effects of a model-AGE and A[beta] on cell viability, ATP level, glucose consumption and lactate production in the neuroblastoma cell line SH-SY5Y. The results show that AGEs and A[beta] increase glucose consumption and decrease ATP levels in a dose dependent manner. Furthermore, both compounds decrease mitochondrial activity measured by the MTT assay. However, only AGEs decrease the number of cells and significantly increase lactate production. These data indicate that both AGEs and A[beta] can cause differential disturbances in neuronal metabolism, which may contribute to the pathophysiological findings in Alzheimer's disease. However, their signalling pathways are apparently quite distinct, a fact which should stimulate a more detailed investigation in this field, e.g. for the purpose of a rational design of potential "neuroprotective" RAGE antagonists.