Isolation and biochemical mapping in the range of 10 −7 to 10 −12 g of fresh, single mammalian neurons in brain II. Some applications

• Published in: TrAC, Trends in analytical chemistry (Regular ed.) Vol. 14; no. 4; pp. 148 - 154
• Main Author: HYDEN, H
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 01-04-1995; Elsevier Science
• Subjects: Biological and medical sciences; Fundamental and applied biological sciences. Psychology; Isolated neuron and nerve. Neuroglia; Vertebrates: nervous system and sense organs; RNA; Memory; Neuroglia; Central nervous system; Review; Gene expression; Learning; Vertebrata; Nucleic base; Mammalia; Neuron; Acquisition process; Protein synthesis; Chemical composition; Brain (vertebrata)
• ISSN: 0165-9936; 1879-3142
• DOI: 10.1016/0165-9936(95)98311-U

Some applications and results are given from studies on: RNA base ratio changes and gene activation and specific protein synthesis during acquisition of new behavior in rats, rabbits and Rhesus monkeys, on glia changes during training to learn, the neuron-glia relationship, and on analysis of the mechanism of neuronal inhibition by GABA receptors, using fresh plasma membranes. Future directions are discussed. Studies on fresh, single nerve cells and glia using the micromethods are described.