Comparison of Allergic Peripheral Lung and Airway Tissue Inflammation

Comparison of Allergic Peripheral Lung and Airway Tissue Inflammation

Tissue was cut into 2mm3 explants and stimulated either with anti-IgE, LPS or buffer control for 24 hours at 37°C. Tissue was harvested, weighed and supernatants aliquoted and stored at -80°C. Supernatants were analysed for TNFα and IL-10 using ELISA.

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Bibliographic Details
Published in:Journal of allergy and clinical immunology Vol. 125; no. 2; p. AB48
Main Authors: Rankin, G.D, Warner, J.A, Hall, D.A
Format: Journal Article
Language:English
Published: St. Louis Mosby, Inc 01-02-2010
Elsevier Limited
Subjects:
Allergy and Immunology
Online Access:Get full text
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Description
Summary:Tissue was cut into 2mm3 explants and stimulated either with anti-IgE, LPS or buffer control for 24 hours at 37°C. Tissue was harvested, weighed and supernatants aliquoted and stored at -80°C. Supernatants were analysed for TNFα and IL-10 using ELISA.
ISSN:0091-6749
1097-6825
DOI:10.1016/j.jaci.2009.12.221