A comparison of cytosolic free Ca2+ in resting feline and rat ventricular myocytes

The Ca2+-sensitive dye quin-2 was used to measure the cytosolic free Ca2+ (Cai2+) in suspensions of ventricular myocytes isolated from cat and rat ventricles. Following an isolation procedure that was similar for both species, the cells were loaded with quin-2 AM (25 microM) for 30 min at 37 degrees...

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Bibliographic Details
Published in:	Cell calcium (Edinburgh) Vol. 8; no. 4; p. 259
Main Authors:	duBell, W H, Houser, S R
Format:	Journal Article
Language:	English
Published:	Netherlands 01-08-1987
Subjects:	Aminoquinolines Animals Calcium - metabolism Cats Cytosol - metabolism Fluorescent Dyes Heart Ventricles Myocardium - metabolism Rats Spectrometry, Fluorescence
Online Access:	Get more information
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Summary:	The Ca2+-sensitive dye quin-2 was used to measure the cytosolic free Ca2+ (Cai2+) in suspensions of ventricular myocytes isolated from cat and rat ventricles. Following an isolation procedure that was similar for both species, the cells were loaded with quin-2 AM (25 microM) for 30 min at 37 degrees C. After two washes to remove extracellular dye, the cells were resuspended for fluorescence measurements. Extracellular Ca2+ was 2.0 mM. Resting Cai2+ in the rat (121 +/- 11 nM) was found to be significantly higher than in the cat (57 +/- 4 nM). These results are discussed in terms of known differences in excitation-contraction coupling between these two species.
ISSN:	0143-4160
DOI:	10.1016/0143-4160(87)90002-9