Getting Started with In Situ Cryo-Electron Tomography

• Published in: Methods in molecular biology (Clifton, N.J.) Vol. 2215; p. 3
• Main Authors: Serwas, Daniel, Davies, Karen M
• Format: Journal Article
• Language: English
• Published: United States 2021
• Subjects: Cell Culture Techniques; Cell Line; Cryoelectron Microscopy - methods; Electron Microscope Tomography - methods; Humans; Imaging, Three-Dimensional; Tilt series; Cellular morphology; Segmentation; Vitrification; Cell biology; Cryo-electron tomography; Structural cell biology; Mammalian cells
• ISSN: 1940-6029
• DOI: 10.1007/978-1-0716-0966-8_1

Cryo-electron tomography (cryo-ET) is an extremely powerful tool which is used to image cellular features in their close-to-native environment at a resolution where both protein structure and membrane morphology can be revealed. Compared to conventional electron microscopy methods for biology, cryo-ET does not include the use of potentially artifact generating agents for sample fixation or visualization. Despite its obvious advantages, cryo-ET has not been widely adopted by cell biologists. This might originate from the overwhelming and constantly growing number of complex ways to record and process data as well as the numerous methods available for sample preparation. In this chapter, we will take one step back and guide the reader through the essential steps of sample preparation using mammalian cells, as well as the basic steps involved in data recording and processing. The described protocol will allow the reader to obtain data that can be used for morphological analysis and precise measurements of biological structures in their cellular environment. Furthermore, this data can be used for more elaborate structural analysis by applying further image processing steps like subtomogram averaging, which is required to determine the structure of proteins.