assessment of cultivated emmer germplasm for gluten proteins

• Published in: Euphytica Vol. 145; no. 1-2; pp. 49 - 55
• Main Authors: Degaonkar, A.M, Tamhankar, S.A, Rao, V.S
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Nature B.V 01-09-2005
• Subjects: alleles; biochemical polymorphism; Crops; Cultivars; germplasm; germplasm evaluation; gliadin; Gluten; glutenins; high molecular weight glutenins; loci; low molecular weight glutenins; plant genetic resources; polyacrylamide gel electrophoresis; Proteins; Triticum dicoccum; Triticum turgidum subsp. dicoccon
• ISSN: 0014-2336; 1573-5060
• DOI: 10.1007/s10681-005-0142-5

The storage protein composition of 61 accessions of Triticum dicoccum was analyzed by SDS-PAGE (HMW- and LMW-glutenin subunits) and Acid-PAGE (gliadins). In the HMW-glutenin subunits, four allelic variants at the Glu-A1 and eight at the Glu-B1 locus were detected resulting in a total of 17 patterns. The Glu-B1 locus was found to be more polymorphic than the Glu-A1 locus. Interestingly, the presence of HMW subunits like 13+16, 2^sup ^ and 1 associated with good quality was observed. Three accessions were null for both the Glu-A1 and Glu-B1 loci. There was less variation for gliadins. Three different gamma gliadin fractions coded by Gli-B1 locus were detected and there were eight different LMW-B glutenin patterns at the Glu-3 loci. The variability can be used to improve the utility of this crop.[PUBLICATION ABSTRACT]