Lymphocyte chimerism after bone marrow transplantation. Surface markers and in vitro function of donor and recipient lymphocyte subpopulations

• Published in: Scandinavian journal of immunology Vol. 6; no. 4; p. 299
• Main Authors: Hansen, G S, Dupont, B, Faber, V, Jakobsen, B K, Juhl, F, Nielsen, L S, Svejgaard, A, Thomsen, M, Wiik, A
• Format: Journal Article
• Language: English
• Published: England 1977
• Subjects: B-Lymphocytes - immunology; Bone Marrow Cells; Bone Marrow Transplantation; Cell Survival; Cells, Cultured; Child; Complement System Proteins; Histocompatibility Testing; HLA Antigens; Humans; Immune Adherence Reaction; Immunologic Deficiency Syndromes - therapy; Lymphocyte Activation; Lymphocytes - immunology; Male; Receptors, Antigen, B-Cell - analysis; T-Lymphocytes - immunology; Transplantation, Homologous
• ISSN: 0300-9475
• DOI: 10.1111/j.1365-3083.1977.tb00397.x

Specific HLA antibodies were used to eliminate donor and recipient cells, respectively, from lymphocyte suspensions prepared from the blood of a child who had been transplanted with bone marrow from an HLA-A- and HLA-B-incompatible, HLA-D-compatible donor. About 70% of the lymphocytes were of donor HLA type, the remaining of recipient type. The phytohemagglutinin-responsive lymphocytes were exclusively limited to the lymphocyte population carrying donor-type HLA antigens. Membrane immunofluorescence investigations of the donor and recipient populations showed a low percentage of IgM-positive lymphocytes in the donor population and an extremely high proportion of IgM-positive lymphocytes in the recipient population. About 90% of the donor lymphocytes were T cells, as judged by their capacity to form rosettes between sheep erythrocytes and T lymphocytes; no cells in the recipient cell population expressed this ability.