Ascite-solid model of prostate cancer and its morphological characteristics

• Published in: Sibirskiĭ onkologicheskiĭ zhurnal Vol. 22; no. 4; pp. 65 - 73
• Main Authors: Plotnikova, E. A., Morozova, N. B., Ryabova, A. V., Trunova, G. V., Khokhlova, V. A., Loschenov, V. B., Pankratov, A. A., Kaprin, A. D.
• Format: Journal Article
• Language: English; Russian
• Published: Russian Academy of Sciences, Tomsk National Research Medical Center 06-09-2023
• Subjects: ascite-solid prostate cancer model; prostate cancer; prostate specific membrane antigen; tramp-c2 cell line
• ISSN: 1814-4861; 2312-3168
• DOI: 10.21294/1814-4861-2023-22-4-65-73

Targeted therapy and immunotherapy are considered promising novel therapies capable of increasing the effcacy of prostate cancer (PCa) treatment.
The purpose of the study
was to obtain and characterize TRAMP-C2 subcutaneous and ascite-solid models of prostate cancer in C57Bl/6j mice to study specifc anti-tumor activity of the candidate molecules of targeted drugs and adjust immunotherapy strategies in an evidence-based manner.
Material and Methods
. We used cultured TRAMP-C2 cells in subcutaneous and ascites mouse prostate cancer models. Histological and immunohistochemical methods were used to study the tumor tissues.
Results
. The high
in vivo
growth ability of TRAMP-C2 cells was demonstrated in subcutaneous and intraperitoneal inoculation of C57Bl/6j mice. These tumors are characterized by a high reproducibility and level of PSMA expression. Histological study showed that subcutaneous and carcinomatous TRAMP-C2 tumor nodules had solid structure morphologically corresponding to low differentiated neoplasm, cytomorphological analysis of smears showed that peritoneal fuid containd a large number of rounded tumor cells, macrophages and erythrocytes.
Conclusion
. The obtained subcutaneous and ascite-solid models of TRAMP-C2 can be useful for the development of new ways to effectively treat cancer, including targeted and immunotherapy, as well as for the experimental study of biotherapeutic effects using PSMA as a target, and photoinduced effects.