A new human breast carcinoma cell line (PMC42) with stem cell characteristics. I. Morphologic characterization

A new human breast carcinoma cell line (PMC42) was established from a pleural effusion from a woman with metastatic breast cancer. The cells were significantly pleomorphic even after 3.5 years in continuous culture. Eight different cell types could be characterized morphologically in monolayer cultu...

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Bibliographic Details
Published in:	JNCI : Journal of the National Cancer Institute Vol. 70; no. 4; p. 649
Main Authors:	Whitehead, R H, Bertoncello, I, Webber, L M, Pedersen, J S
Format:	Journal Article
Language:	English
Published:	United States 01-04-1983
Subjects:	Aged Breast Neoplasms - pathology Breast Neoplasms - ultrastructure Cell Differentiation Cell Division Cell Line Clone Cells Culture Techniques - methods Female Humans Microscopy, Electron
Online Access:	Get more information
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Summary:	A new human breast carcinoma cell line (PMC42) was established from a pleural effusion from a woman with metastatic breast cancer. The cells were significantly pleomorphic even after 3.5 years in continuous culture. Eight different cell types could be characterized morphologically in monolayer culture. Cells cloned in agar and replated in monolayer culture were equally heterogenous. Cells also grew in suspension in papillary clusters that structurally resembled glandular organoids. Electron microscopy confirmed the differentiated structure of these cells in which many of the features of lactating breast tissue were evident. It is proposed that this is a culture derived from a malignant breast stem that has retained its ability to differentiate in vitro.
ISSN:	0027-8874
DOI:	10.1093/jnci/70.4.649