Four cutaneous leishmaniosis case resistant to meglumine antimoniate treatment

• Published in: Türkiye parazitolojii dergisi Vol. 38; no. 3; pp. 177 - 180
• Main Authors: Polat, Erdal, Kutlubay, Zekayi
• Format: Journal Article
• Language: English; Turkish
• Published: Turkey Galenos Publishing House 2014
• Subjects: Amphotericin B - therapeutic use; Animals; Antiprotozoal Agents - therapeutic use; Diptera - physiology; DNA, Intergenic - isolation & purification; DNA, Protozoan - isolation & purification; Drug Resistance; Humans; Larva; Leishmania; Leishmania major - drug effects; Leishmania major - genetics; Leishmania major - pathogenicity; Leishmaniasis, Cutaneous - parasitology; Leishmaniasis, Cutaneous - therapy; Meglumine - therapeutic use; Organometallic Compounds - therapeutic use; Treatment Outcome
• ISSN: 1300-6320; 2146-3077
• DOI: 10.5152/tpd.2014.3410

The aim of the study was to treatment of cutaneous leishmaniasis caused by meglumine antimoniate resistant Leishmania major with Lucilia sericata larvae.
Samples obtained from patients'lesions were stained with Giemsa and examined under the microscope. The samples were also incubated to Novy-Nicolle-McNeal (NNN) and Roswell Park Memorial Institute (RPMI) 1640 media. ITS1 region real time PCR was performed for identfying the Leishmania species. The patients were treated using Glucantime® or liposomal amphotericin B or L. sericata larvae according to their response to the treatmet regimes.
In the initial examination, amastigote forms of Leishmania were seen in the samples of all 4 patients under microscopic examination. Two of the patients with cutaneous leishmaniasis, which were caused by Glucantime resistant L. major were treated with liposomal amphotericin B, and two of them were treated with L. sericata larvae. Leishmania promastigotes were not grown in both cultures.
Cutaneous leishmaniasis, which wascaused by Glucantime resistant L. major has been successfully treated with L. sericata larvae in a very short time, 10 days. PCR results from samples taken 2 months after the treatment were examined and the results were negative.