Transformation of Maize Using Microprojectile Bombardment: An Update and Perspective

• Published in: In vitro cellular & developmental biology. Plant Vol. 27P; no. 1; pp. 21 - 27
• Main Authors: W. J. Gordon-Kamm, T. M. Spencer, J. V. O'Brien, W. G. Start, R. J. Daines, T. R. Adams, M. L. Mangano, Chambers, S. A., S. J. Zachwieja, N. G. Willetts, W. R. Adams, Jr, C. J. Mackey, R. W. Krueger, A. P. Kausch, P. G. Lemaux
• Format: Journal Article
• Language: English
• Published: Tissue Culture Association, Inc 01-01-1991
• Subjects: Callus; Corn; DNA; Plant cells; Plant fertility; Plants; Pollen; Protoplasts; Rice; Transgenic plants
• ISSN: 1054-5476; 1475-2689
• DOI: 10.1007/BF02632057

Using microprojectile bombardment of maize suspension cultures and bialaphos selection, transformed embryogenic calli have been recovered in numerous independent experiments. Fertile transgenic plants have been regenerated from several transformed callus lines. Stable inheritance and expression of bar and functional activity of the enzyme phosphinothricin acetyl transferase were observed in three subsequent generations of transformed plants. Evidence to date indicates that the transformation process and the presence of the foreign gene per se do not detrimentally influence either plant vigor or fertility. This represents a practical method for introducing foreign genes into maize, which may be applicable to other monocot species.