SKIP Downregulation Increases TGF- β 1-Induced Matrix Metalloproteinase-9 Production in Transformed Keratinocytes
Transforming growth factor-beta (TGF- β 1) is a potent inductor of matrix metalloproteinase-9 (MMP-9) in transformed cells. Recently, Ski-interacting protein (SKIP) has been described as a regulator of TGF- β 1 signal transduction, but its role in the induction of cell malignance by TGF- β 1 has not...
Saved in:
| Published in: | Scientifica (Cairo) Vol. 2012; pp. 1 - 8 |
|---|---|
| Main Authors: | , , , |
| Format: | Journal Article |
| Language: | English |
| Published: |
2012
|
| Online Access: | Get full text |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Abstract | Transforming growth factor-beta (TGF-
β
1) is a potent inductor of matrix metalloproteinase-9 (MMP-9) in transformed cells. Recently, Ski-interacting protein (SKIP) has been described as a regulator of TGF-
β
1 signal transduction, but its role in the induction of cell malignance by TGF-
β
1 has not been fully elucidated so far. In the present study, we analyzed the role of SKIP on TGF-
β
1-induced MMP-9 production. Mouse transformed keratinocytes (PDV) were stably transfected with SKIP antisense construct. We observed that SKIP depletion provoked an enhancement in the expression of MMP-9 in response to TGF-
β
1 treatment. The downregulation of SKIP produced an enhancement in TGF-
β
1-activated ERK1,2 MAP kinase as well as increased transactivation of downstream Elk1 transcription factor. The increased MMP-9 production in response to TGF-
β
1 was dependent of MAPK activation as PD98059, an MEK inhibitor, reduced MMP-9 expression in SKIP antisense transfected cells. Thus, we propose SKIP as a regulatory protein in TGF-
β
1-induced MMP-9 expression acting by controlling ERK1,2 signaling in transformed cells. |
|---|---|
| AbstractList | Transforming growth factor-beta (TGF-
β
1) is a potent inductor of matrix metalloproteinase-9 (MMP-9) in transformed cells. Recently, Ski-interacting protein (SKIP) has been described as a regulator of TGF-
β
1 signal transduction, but its role in the induction of cell malignance by TGF-
β
1 has not been fully elucidated so far. In the present study, we analyzed the role of SKIP on TGF-
β
1-induced MMP-9 production. Mouse transformed keratinocytes (PDV) were stably transfected with SKIP antisense construct. We observed that SKIP depletion provoked an enhancement in the expression of MMP-9 in response to TGF-
β
1 treatment. The downregulation of SKIP produced an enhancement in TGF-
β
1-activated ERK1,2 MAP kinase as well as increased transactivation of downstream Elk1 transcription factor. The increased MMP-9 production in response to TGF-
β
1 was dependent of MAPK activation as PD98059, an MEK inhibitor, reduced MMP-9 expression in SKIP antisense transfected cells. Thus, we propose SKIP as a regulatory protein in TGF-
β
1-induced MMP-9 expression acting by controlling ERK1,2 signaling in transformed cells. |
| Author | Krstić, Aleksandra Kocić, Jelena Villar, Victor Santibanez, Juan F. |
| Author_xml | – sequence: 1 givenname: Jelena surname: Kocić fullname: Kocić, Jelena organization: Laboratory for Experimental Hematology, Institute for Medical Research, University of Belgrade, Dr. Subotića 4, P.O. Box 102, 11129 Belgrade, Serbia, Department of Biology, University of the Balearic Islands, Ctrretera Valldemossa, Km 7.5, 07122 Palma de Mallorca, Spain – sequence: 2 givenname: Victor surname: Villar fullname: Villar, Victor organization: Laboratorio de Biología Celular, Instituto de Nutrición y Tecnología de los Alimentos, Universidad de Chile, Santiago, Chile – sequence: 3 givenname: Aleksandra surname: Krstić fullname: Krstić, Aleksandra organization: Laboratory for Experimental Hematology, Institute for Medical Research, University of Belgrade, Dr. Subotića 4, P.O. Box 102, 11129 Belgrade, Serbia – sequence: 4 givenname: Juan F. surname: Santibanez fullname: Santibanez, Juan F. organization: Laboratory for Experimental Hematology, Institute for Medical Research, University of Belgrade, Dr. Subotića 4, P.O. Box 102, 11129 Belgrade, Serbia, Laboratorio de Biología Celular, Instituto de Nutrición y Tecnología de los Alimentos, Universidad de Chile, Santiago, Chile |
| BookMark | eNpNkEFOwzAQRS1UJErpigt4j0JnnNROlqjQErUVlQgSu8hxbBSU2pWdCnotDsKZSCkLZjMj_T9v8S7JwDqrCblGuOXAkwkDZJOUI0_EGRkyyCDKIH0d_LsvyDiEd-hHIMMUhsQ_L_MNvXcf1uu3fSu7xlmaW-W1DDrQYjGP6PcXxSi39V7pmq5l55tPutadbFu3867Tje27UUY33vWdX0JjaeGlDcb5bf-01L4nW6cOnQ5X5NzINujx3x6Rl_lDMXuMVk-LfHa3ihTyuIsylklesdokWDGMK54gCgOJzsCkaMRUpFKZKVaCcaVUHwpgPIlrxtIpQBWPyM2Jq7wLwWtT7nyzlf5QIpRHY-XRWHkyFv8Ad_dgAg |
| CitedBy_id | crossref_primary_10_3389_fonc_2019_00234 |
| Cites_doi | 10.1016/S0959-437X(01)00259-3 10.1096/fasebj.13.8.781 10.1007/s10555-006-7886-9 10.1038/sj.ejhg.5201034 10.1073/pnas.1633291100 10.1016/j.ejca.2011.06.043 10.1016/S0006-291X(02)00864-1 10.1038/sj.onc.1201687 10.1016/j.jsbmb.2004.03.097 10.1016/j.bbrc.2008.04.128 10.1016/j.febslet.2010.10.020 10.1189/jlb.71.5.731 10.1002/jcp.20311 10.1016/j.bbrc.2009.04.038 10.1002/glia.20637 10.1016/S0092-8674(03)00432-X 10.1038/nature02006 10.1038/cr.2008.316 10.4049/jimmunol.173.3.2099 10.1074/jbc.M010815200 10.1007/s00018-003-3215-4 |
| ContentType | Journal Article |
| DBID | AAYXX CITATION |
| DOI | 10.6064/2012/861647 |
| DatabaseName | CrossRef |
| DatabaseTitle | CrossRef |
| DatabaseTitleList | CrossRef |
| DeliveryMethod | fulltext_linktorsrc |
| Discipline | Biology |
| EISSN | 2090-908X |
| EndPage | 8 |
| ExternalDocumentID | 10_6064_2012_861647 |
| GroupedDBID | 24P 5VS AAFWJ AAJEY AAYXX ABDBF ADBBV ADRAZ AFPKN ALMA_UNASSIGNED_HOLDINGS AOIJS BAWUL BCNDV CITATION DIK EBS EJD ESX GROUPED_DOAJ H13 HYE IAO IHR IPNFZ ITC KQ8 M48 M~E OK1 PGMZT RHX RIG RPM |
| ID | FETCH-LOGICAL-c163t-929a6b2df41b213b64117f04e90f81f7578acf51b726ccc117702643d228500b3 |
| ISSN | 2090-908X |
| IngestDate | Thu Nov 21 21:45:24 EST 2024 |
| IsDoiOpenAccess | false |
| IsOpenAccess | true |
| IsPeerReviewed | true |
| IsScholarly | true |
| Language | English |
| LinkModel | OpenURL |
| MergedId | FETCHMERGED-LOGICAL-c163t-929a6b2df41b213b64117f04e90f81f7578acf51b726ccc117702643d228500b3 |
| PageCount | 8 |
| ParticipantIDs | crossref_primary_10_6064_2012_861647 |
| PublicationCentury | 2000 |
| PublicationDate | 2012-00-00 |
| PublicationDateYYYYMMDD | 2012-01-01 |
| PublicationDate_xml | – year: 2012 text: 2012-00-00 |
| PublicationDecade | 2010 |
| PublicationTitle | Scientifica (Cairo) |
| PublicationYear | 2012 |
| References | 22 23 24 25 (10) 2006; 11 (16) 1998; 16 (12) 2002; 18 (13) 2004; 173 (9) 1996; 56 11 14 15 17 18 (2) 2002; 71 19 (8) 1999; 13 1 3 4 5 7 (6) 1997; 80 20 21 |
| References_xml | – ident: 5 doi: 10.1016/S0959-437X(01)00259-3 – volume: 13 start-page: 781 issue: 8 year: 1999 ident: 8 publication-title: FASEB Journal doi: 10.1096/fasebj.13.8.781 – ident: 7 doi: 10.1007/s10555-006-7886-9 – ident: 14 doi: 10.1038/sj.ejhg.5201034 – ident: 4 doi: 10.1073/pnas.1633291100 – ident: 25 doi: 10.1016/j.ejca.2011.06.043 – volume: 11 start-page: 1199 issue: 2 year: 2006 ident: 10 publication-title: Frontiers in Bioscience – ident: 11 doi: 10.1016/S0006-291X(02)00864-1 – volume: 16 start-page: 1579 issue: 12 year: 1998 ident: 16 publication-title: Oncogene doi: 10.1038/sj.onc.1201687 – ident: 17 doi: 10.1016/j.jsbmb.2004.03.097 – ident: 23 doi: 10.1016/j.bbrc.2008.04.128 – ident: 24 doi: 10.1016/j.febslet.2010.10.020 – volume: 71 start-page: 731 issue: 5 year: 2002 ident: 2 publication-title: Journal of Leukocyte Biology doi: 10.1189/jlb.71.5.731 – ident: 19 doi: 10.1002/jcp.20311 – volume: 80 start-page: 1529 issue: 8 year: 1997 ident: 6 publication-title: Cancer – ident: 22 doi: 10.1016/j.bbrc.2009.04.038 – ident: 21 doi: 10.1002/glia.20637 – volume: 56 start-page: 190 issue: 1 year: 1996 ident: 9 publication-title: Cancer Research – ident: 1 doi: 10.1016/S0092-8674(03)00432-X – ident: 3 doi: 10.1038/nature02006 – ident: 20 doi: 10.1038/cr.2008.316 – volume: 173 start-page: 2099 issue: 3 year: 2004 ident: 13 publication-title: Journal of Immunology doi: 10.4049/jimmunol.173.3.2099 – ident: 15 doi: 10.1074/jbc.M010815200 – ident: 18 doi: 10.1007/s00018-003-3215-4 – volume: 18 start-page: 684 year: 2002 ident: 12 publication-title: Chinese Journal of Biochemistry and Molecular Biology |
| SSID | ssj0000712180 |
| Score | 1.8407983 |
| Snippet | Transforming growth factor-beta (TGF-
β
1) is a potent inductor of matrix metalloproteinase-9 (MMP-9) in transformed cells. Recently, Ski-interacting protein... |
| SourceID | crossref |
| SourceType | Aggregation Database |
| StartPage | 1 |
| Title | SKIP Downregulation Increases TGF- β 1-Induced Matrix Metalloproteinase-9 Production in Transformed Keratinocytes |
| Volume | 2012 |
| hasFullText | 1 |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| link | http://sdu.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwtV1LbxMxELbSVkhcEFAQr1Y-tKfI7XrX3ewe-0igREWVklbcIj-rFWiL8hD0b_FD-E3M2Btni3Ioh142ibNjRTtfZj6PxzOE7MkiK0SZG_iL24IJqTQDElswJ7G2uclgzeGb2I56X74WZ33R73SWHQhXY4-qaRgDXePJ2f_QdpwUBuA96ByuoHW4Pkjvo-H5JdDin_D1TdOaC40A5p7bWXf8ccC6-6f9_ZO0yxn27cD9_wus0_-re2HnuA3vSzdUNdzPSjxIYEKBWYyMjJc0F4SGWI65qm_13bzJQ2w4rjcXPgVJ-j1iWeFZmlVSN8DBU9heyK4BtxddwzX2QPIYuq50qIMcZICjtoSOv9tvM1mbaRQcAUAqJesQD_-8AKs1OGhHNHgrvJkmZcLKxDcbBu-0Zqyx2VEsmF3e9t_rHAMs0wSefQExeClyrKK28oDLXf9_HGNMV4SFEk4wQfFJEN4gWymYNuyZcXY-jFE9IGzAmTCwF392OBSK8ocofxjkWzSoxWfGz8mzZiFCjwOCXpCOrV-SJ6E16d02mSKO6H0c0Ygjijiif37TiCEaMETXYIiuMESrmrYwRO9h6BW5GvTHp59Y05-DaWDxcwbMWuYqNU5wlfJM5YLznkuELRNXcIedEqR2R1z10lxrjekBsOIXmUnT4ihJVPaabNa3tX1DqBAGJsiUsfBwjOBSCwVDwmXcWeuyt2Rv-bgmP0IZlskarbx72G3vyVP8FCJpH8jmfLqwO2RjZha7Xp27PjLzF0u9d80 |
| link.rule.ids | 315,782,786,866,4028,27932,27933,27934 |
| linkProvider | Flying Publisher |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=SKIP+Downregulation+Increases+TGF-+%CE%B2+1-Induced+Matrix+Metalloproteinase-9+Production+in+Transformed+Keratinocytes&rft.jtitle=Scientifica+%28Cairo%29&rft.au=Koci%C4%87%2C+Jelena&rft.au=Villar%2C+Victor&rft.au=Krsti%C4%87%2C+Aleksandra&rft.au=Santibanez%2C+Juan+F.&rft.date=2012&rft.issn=2090-908X&rft.eissn=2090-908X&rft.volume=2012&rft.spage=1&rft.epage=8&rft_id=info:doi/10.6064%2F2012%2F861647&rft.externalDBID=n%2Fa&rft.externalDocID=10_6064_2012_861647 |
| thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=2090-908X&client=summon |
| thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=2090-908X&client=summon |
| thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=2090-908X&client=summon |