SKIP Downregulation Increases TGF- β 1-Induced Matrix Metalloproteinase-9 Production in Transformed Keratinocytes

• Published in: Scientifica (Cairo) Vol. 2012; pp. 1 - 8
• Main Authors: Kocić, Jelena, Villar, Victor, Krstić, Aleksandra, Santibanez, Juan F.
• Format: Journal Article
• Language: English
• Published: 2012
• ISSN: 2090-908X; 2090-908X
• DOI: 10.6064/2012/861647

Transforming growth factor-beta (TGF-
β
1) is a potent inductor of matrix metalloproteinase-9 (MMP-9) in transformed cells. Recently, Ski-interacting protein (SKIP) has been described as a regulator of TGF-
β
1 signal transduction, but its role in the induction of cell malignance by TGF-
β
1 has not been fully elucidated so far. In the present study, we analyzed the role of SKIP on TGF-
β
1-induced MMP-9 production. Mouse transformed keratinocytes (PDV) were stably transfected with SKIP antisense construct. We observed that SKIP depletion provoked an enhancement in the expression of MMP-9 in response to TGF-
β
1 treatment. The downregulation of SKIP produced an enhancement in TGF-
β
1-activated ERK1,2 MAP kinase as well as increased transactivation of downstream Elk1 transcription factor. The increased MMP-9 production in response to TGF-
β
1 was dependent of MAPK activation as PD98059, an MEK inhibitor, reduced MMP-9 expression in SKIP antisense transfected cells. Thus, we propose SKIP as a regulatory protein in TGF-
β
1-induced MMP-9 expression acting by controlling ERK1,2 signaling in transformed cells.