trans Activation of Gene Expression by v-myb
The v-myb oncogene causes acute myelomonocytic leukemia in chickens and transforms avian myeloid cells in vitro. Its product, p48 v- myb , is a short-lived nuclear protein which binds DNA. We demonstrate that p48 v- myb can function as a trans activator of gene expression in transient DNA transfecti...
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| Published in: | Molecular and cellular biology Vol. 10; no. 5; pp. 2285 - 2293 |
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| Main Authors: | , |
| Format: | Journal Article |
| Language: | English |
| Published: |
Taylor & Francis
01-05-1990
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| Online Access: | Get full text |
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| Summary: | The v-myb oncogene causes acute myelomonocytic leukemia in chickens and transforms avian myeloid cells in vitro. Its product, p48
v-
myb
, is a short-lived nuclear protein which binds DNA. We demonstrate that p48
v-
myb
can function as a trans activator of gene expression in transient DNA transfection assays, trans activation requires the highly conserved amino-terminal DNA-binding domain and the less highly conserved carboxyl-terminal domain of p48
v-
myb
, both of which are required for transformation. Multiple copies of a consensus sequence for DNA binding by p48
v-
myb
inserted upstream of a herpes simplex virus thymidine kinase promoter are strongly stimulatory for transcriptional activation by a v-myb-VP16 fusion protein but not by p48
v-
myb
itself, suggesting that the binding of p48
v-
myb
to DNA may not be sufficient for trans activation. |
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| ISSN: | 1098-5549 1098-5549 |
| DOI: | 10.1128/mcb.10.5.2285-2293.1990 |