Kurloff cell proteoglycans

Kurloff cell proteoglycans

This paper reports the first direct demonstration of de novo synthesis ofchondroitin sulphate proteoglycans by Kurloff cells. This was achieved using highly purified splenic Kurloff cells labelled in vitro with [35S]sulphate and D‐[U‐3H]glucosamine. A single population of sulphated proteoglycans was...

Full description

Saved in:
Bibliographic Details
Published in:FEBS letters Vol. 209; no. 2; pp. 299 - 304
Main Authors: Landemore, Gérard, Letaïef, Slim-Eric, Bocquet, Jean, Izard, Jacques
Format: Journal Article
Language:English
Published: 15-12-1986
Subjects:
Chondroitin sulfate
Glycosaminoglycan
Kurloff cell, Spleen cell
Leukocyte
Proteoglycan
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:This paper reports the first direct demonstration of de novo synthesis ofchondroitin sulphate proteoglycans by Kurloff cells. This was achieved using highly purified splenic Kurloff cells labelled in vitro with [35S]sulphate and D‐[U‐3H]glucosamine. A single population of sulphated proteoglycans was observed after dissociative extraction, DEAE‐cellulose chromatography, Sepharose CL 6B chromatography and fluorography after electrophoresis. These were large, highly anionic proteoglycans and were completely digested by chondroitinase AC or ABC. Moreover, glycosaminoglycan extracted from Kurloff cells had the electrophoretic mobility of control chondroitin sulphate.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(86)81131-0