Enterovirus Immunizing Antigens in the from of Cation-Stabilized and Concentrated Virus Preparations
Summary A method for preparation of concentrated enteroviral antigens is described. Heating virus at 50°C in M MgCl2 inactivates adventitious simian agents. Addition of 25 mM AlCl3 and 50 mM Na2CO3 to the heated, stabilized virus produces a precipitate which selectively adsorbs enteroviruses. The se...
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| Published in: | The Journal of immunology (1950) Vol. 95; no. 1; pp. 9 - 12 |
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| Main Authors: | , , |
| Format: | Journal Article |
| Language: | English |
| Published: |
Am Assoc Immnol
01-07-1965
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| Online Access: | Get full text |
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| Summary: | Summary
A method for preparation of concentrated enteroviral antigens is described. Heating virus at 50°C in M MgCl2 inactivates adventitious simian agents. Addition of 25 mM AlCl3 and 50 mM Na2CO3 to the heated, stabilized virus produces a precipitate which selectively adsorbs enteroviruses. The sediment is reconstituted in one tenth the original volume of virus. Guinea pigs injected with cation-precipitated antigens produced a higher antibody response than animals receiving untreated antigens. |
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| ISSN: | 0022-1767 1550-6606 |
| DOI: | 10.4049/jimmunol.95.1.9 |