Induction of Gαq-specific Antisense RNA in Vivo Causes Increased Body Mass and Hyperadiposity

• Published in: The Journal of biological chemistry Vol. 272; no. 7; pp. 4335 - 4341
• Main Authors: Galvin-Parton, Patricia A., Chen, Xiaohui, Moxham, Christopher M., Malbon, Craig C.
• Format: Journal Article
• Language: English
• Published: Elsevier Inc 14-02-1997
• Subjects: ANIMAL TRANSGENIQUE; ANIMALES TRANSGENICOS; ARN; CELLULE; CELULAS; ESTERASAS; ESTERASE; EXPRESION GENICA; EXPRESSION DES GENES; GAIN DE POIDS; GANANCIA DE PESO; INHIBICION; INHIBITION; LIPOLISIS; LIPOLYSE; NUCLEOTIDE; NUCLEOTIDOS; PESO; POIDS; PROTEINA QUINASA; PROTEINAS AGLUTINANTES; PROTEINE DE LIAISON; PROTEINE KINASE; RATON; RECEPTEUR D'HORMONE; RECEPTORES DE HORMONAS; SOURIS; TEJIDO ADIPOSO; TISSU ADIPEUX
• ISSN: 0021-9258; 1083-351X
• DOI: 10.1074/jbc.272.7.4335

Transgenic BDF-1 mice harboring an inducible, tissue-specific transgene for RNA antisense to Gαq provide a model in which to study a loss-of-function mutant of Gαqin vivo Gαq deficiency induced in liver and white adipose tissue at birth produced increased body mass and hyperadiposity within 5 weeks of birth that persisted throughout adult life. Gαq-deficient adipocytes display reduced lipolytic responses, shown to reflect a newly discovered, α1-adrenergic regulation of lipolysis. This α1-adrenergic response via phosphoinositide hydrolysis and activation of protein kinase C is lacking in the Gαq loss-of-function mutants in vivo and provides a basis for the increased fat accumulation.