Mutations Including IS 6110 Insertion in the Gene Encoding the MPB64 Protein of Capilia TB-Negative Mycobacterium tuberculosis Isolates

Mutations Including IS 6110 Insertion in the Gene Encoding the MPB64 Protein of Capilia TB-Negative Mycobacterium tuberculosis Isolates

A simple immunochromatographic assay, Capilia TB, using anti-MPB64 monoclonal antibodies, is a kit for discriminating between the Mycobacterium tuberculosis complex and mycobacteria other than tubercle bacilli. The sensitivity of the kit was estimated to be 99.2% (381 of 384 samples). The sequencing...

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Bibliographic Details
Published in:Journal of clinical microbiology Vol. 42; no. 1; pp. 390 - 392
Main Authors: Hirano, Kazue, Aono, Akio, Takahashi, Mitsuyoshi, Abe, Chiyoji
Format: Journal Article
Language:English
Published: 01-01-2004
Online Access:Get full text
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Summary:A simple immunochromatographic assay, Capilia TB, using anti-MPB64 monoclonal antibodies, is a kit for discriminating between the Mycobacterium tuberculosis complex and mycobacteria other than tubercle bacilli. The sensitivity of the kit was estimated to be 99.2% (381 of 384 samples). The sequencing analysis revealed that all of the Capilia TB-negative isolates had mutations within the mpb64 gene, leading to the production of an incomplete protein as a result of a deletion of the C-terminal region of the protein.
ISSN:0095-1137
1098-660X
DOI:10.1128/JCM.42.1.390-392.2004