Polymorphism of spore germination receptor genes in Bacillus anthracis strains of major genetic lineages

• Published in: Žurnal mikrobiologii, ėpidemiologii i immunobiologii Vol. 101; no. 2; pp. 237 - 247
• Main Authors: Eremenko, Evgeny I., Ryazanova, Alla G., Pechkovskii, Grigorii A., Pisarenko, Sergey V., Kovalev, Dmitry A., Aksenova, Lyudmila Yu, Semenova, Olga V., Kulichenko, Alexander N.
• Format: Journal Article
• Language: English; Russian
• Published: Central Research Institute for Epidemiology 10-05-2024
• Subjects: bacillus anthracis; genes and proteins of spore germination receptors; genetic lineages
• ISSN: 0372-9311; 2686-7613
• DOI: 10.36233/0372-9311-483

Introduction. The genetic structure of the global population of Bacillus anthracis characterized by an unequal distribution of isolates of the main genetic lineages A, B and C, the reason for which is unknown. Determining the characteristics of genes encoding factors that determine the existence of this pathogen at the intra- and extra organismal stages of the life cycle, which can influence the prevalence of strains, is relevant.
Aim — сharacterization of the genes and proteins of spore germination in strains of the anthrax pathogen of different genetic lineages.
Materials and methods. Whole genome sequences of 46 B. anthracis strains and the CI strain of B. cereus biovar anthracis from the NCBI GenBank database studied. In silico analysis carried out using the programs “BLASTn”, “MEGA X”, “Tandem Repeat Finder”.
Results. The number of SNPs, indels and pseudogenes in B. anthracis strains of line B was 2,7–25,6 times higher, in line C 2–23,5 times, and in the B. cereus biovar anthracis strain was 20–2841 times higher than in strains of line A. Significant substitutions in genes leading to changes in the amino acid composition of 10 germination receptor proteins were also significantly more common in B. anthracis strains of lines B, C and the B. cereus biovar anthracis strain.
Undescribed VNTRs within the gerHA gene with repeat units of 78 and 117 bp identified, varying between and within isolates of different genetic lineages. Six germination receptor genes have been shown to have rare starting codons.
Conclusion. A larger number of non-synonymous SNPs in the genes of spore germination receptors with changes in the amino acid composition of proteins in B. anthracis strains of the main genetic lines B, C and B. cereus biovar anthracis than in strains of line A suggests their limited adaptive capabilities and may be one of the explanations for the lower prevalence compared to line A. Differences in the gerHA and gerM genes make it possible to differentiate the major B and C genetic lineages from A.