Influence of Helleborus odorus W. et K. underground parts extracts on red blood pictures of Wistar rats

• Published in: Veterinarski glasnik Vol. 64; no. 3-4; pp. 219 - 229
• Main Authors: Davidovic, Vesna, Lazarevic, Miodrag, Joksimovic-Todorovic, Mirjana, Maksimovic, Zoran, Jovanovic, Milan, Miljas, Niko
• Format: Journal Article
• Language: English
• Published: Faculty of Veterinary Medicine, Belgrade 2010
• Subjects: Helleborus sp; rats; red blood cell count parameters
• ISSN: 0350-2457; 2406-0771
• DOI: 10.2298/VETGL1004219D

This paper presents the results of investigations of parameter values of the red blood cell count in Wistar rats, after intramuscular application of rhizome and root extract of Helleborus odorus W. et K (HE). The trial was conducted in experimental conditions on 49 Wistar rats divided into 7 groups with 7 animals per unit (control and six experimental groups). The control group was treated by an intramuscular injection of sterile saline (0.25 ml/100 g BM). For the purpose of determing the duration of the HE effects, the animals were injected with a dose of 10 mg/100 g BMand blood was collected by cardial taps after 24, 48 and 72 hrs. Moreover, we investigated the influence of elevated temperatures on the noted HE extract effects, 24 hrs after the application of the inactivated (56oC 30 min) or denaturized (100oC 5 min) extract, as well as the influence of dialyzed HE. The obtained results of haematological analyses have pointed out the weak hemolyses activity of the extract. In all experimental groups, the erythrocyte count was decreased, but remained within reference values. Haemoglobin concentration and haematocrit values in the experimental and the control groups of rats were decreased in comparison to the physiological values, while the applied extract did not significantly affect the examined parameters. .
U ovom radu su prikazani rezultati ispitivanja vrednosti parametara crvene krvne slike pacova soja Wistar, nakon intramuskularne aplikacije ekstrakta rizoma i korena Helleborus odorus W. et K. Ogled je izveden u eksperimentalnim uslovima na 49 pacova soja Wistar, podeljenih u 7 grupa sa po 7 jedinki (jedna kontrolna i sest oglednih grupa). Kontrolnoj grupi pacova je intramuskularno aplikovan fizioloski rastvor (0,25 ml/100 g t.m.). U cilju odredjivanja duzine trajanja efekata ekstrakta, pacovima je aplikovan tecni ekstrakt podzemnih organa kukureka u koncentraciji od 10 mg/100 g t.m., a krv je uzorkovana kardijalnom punkcijom nakon 24h, 48h i 72h. Ispitivan je i uticaj ekstrakta izlaganog povisenoj temperaturi na uocene efekte, 24h posle aplikacije inaktivisanog (56oC 30 min) ili denaturisanog ekstrakta (100oC 5 min), kao i efekat dijalizata ekstrakta. Dobijeni rezultati hematoloskih analiza su ukazali na slabu hemoliticku aktivnost ekstrakta. Kod svih oglednih grupa, prosecan broj eritrocita bio je smanjen, ali se odrzao u granicama referentnih vrednosti. Zabelezene koncentracije hemoglobina i hematokrita u krvi pacova kontrolne i oglednih grupa bile su nize u odnosu na fizioloske vrednosti, pri cemu korisceni ekstrakt nije znacajno uticao na ispitivane parametre.