Naringenin complexed with hydroxypropyl-β-cyclodextrin improves the sciatic nerve regeneration through inhibition of p75NTR and JNK pathway

• Published in: Life sciences (1973) Vol. 241; p. 117102
• Main Authors: Oliveira, Marlange A., Heimfarth, Luana, Passos, Fabiolla Rocha Santos, Miguel-dos-Santos, Rodrigo, Mingori, Moara R., Moreira, José Cláudio F., Lauton, Sandra S., Barreto, Rosana S.S., Araújo, Adriano A.S., Oliveira, Aldeidia P., Oliveira, Júlia T., Baptista, Abrahao Fontes, Martinez, Ana Maria B., Quintans-Júnior, Lucindo J., Quintans, Jullyana S.S.
• Format: Journal Article
• Language: English
• Published: Elsevier Inc 15-01-2020
• Subjects: Cyclodextrin; Flavonoids; IL-10; Naringenin; p75NTR; Peripheral nerve injury; Regeneration; TNF-α; CD; p75NTR; HPβCD; Naringenin; CNS; Peripheral nerve injury; PNI; IL-1β; IL-10; IL-6; NPs; Regeneration; Flavonoids; Cyclodextrin; NA; TNF-α; SFI; ELISA
• ISSN: 0024-3205; 1879-0631
• DOI: 10.1016/j.lfs.2019.117102

Peripheral nerve injuries are common conditions that often lead to dysfunctions. Although much knowledge exists on the several factors that mediate the complex biological process involved in peripheral nerve regeneration, there is a lack of effective treatments that ensure full functional recovery. Naringenin (NA) is the most abundant flavanone found in citrus fruits and it has promising neuroprotective, anti-inflammatory and antioxidant effects. This study aimed to enhance peripheral nerve regeneration using an inclusion complex containing NA and hydroxypropyl-β-cyclodextrin (HPβCD), named NA/HPβCD. A mouse sciatic nerve crush model was used to evaluate the effects of NA/HPβCD on nerve regeneration. Sensory and motor parameters, hyperalgesic behavior and the sciatic functional index (SFI), respectively, improved with NA treatment. Western blot analysis revealed that the levels of p75NTR ICD and p75NTR full length as well phospho-JNK/total JNK ratios were preserved by NA treatment. In addition, NA treatment was able to decrease levels of caspase 3. The concentrations of TNF-α and IL-1β were decreased in the lumbar spine, on the other hand there was an increase in IL-10. NA/HPβCD presented a better overall morphological profile but it was not able to increase the number of myelinated fibers. Thus, NA was able to enhance nerve regeneration, and NA/HPβCD decreased effective drug doses while maintaining the effect of the pure drug, demonstrating the advantage of using the complex over the pure compound.
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•NA and NA/HPβCD improve the sensory and motor function recovery in PNI model mice.•NA and NA/HPβCD improve the regeneration process in the PNI model mice•NA and NA/HPβCD inhibit p75NTR, MAPK and caspase 3 pathway.•NA/HPβCD complex seems to have better pharmacological properties than NA.•Naringenin might be considered a promising candidate for PNI treatment.