PCR-based genotyping of MNSs blood group: Subtyping of M allele to MG and MT

• Published in: Journal of human genetics Vol. 42; no. 4; pp. 489 - 498
• Main Authors: Akane, Atsushi, Kobayashi, Tetsuya, Li, Zhi-Xiang, Yoshimura, Sumitaka, Okii, Yutaka, Yoshida, Manabu, Tokiyasu, Takuma, Watabiki, Toshimitsu
• Format: Journal Article
• Language: English
• Published: Tokyo Nature Publishing Group 01-12-1997
• Subjects: Alleles; Conformation; Genotyping; Glycophorin B; Restriction fragment length polymorphism
• ISSN: 1434-5161; 1435-232X
• DOI: 10.1007/BF02767025

SummaryPCR-based genotyping of MNSs blood group system was investigated in combination with restriction fragment length polymorphism (RFLP), single-strand conformation polymorphism (SSCP) and allele-specific PCR amplification (ASPA) techniques. M and N alleles are based on three nucleotide substitutions in exon 2 and one base change (G or T) in an intron of glycophorin A locus. The latter single base change was also found among M alleles analyzed in this study, so that M allele appeared to be subdivided into MG and MT. All three alleles, MG, MT and N were identified clearly by RFLP or SSCP analysis following a single amplification. S and s alleles are based on one nucleotide substitution in exon 3 of glycophorin B gene. Genotyping of Ss blood group system was also explored by PCR-SSCP or ASPA analysis, and problems in the methods were discussed.