Troxerutin associated with Agaricus blazei Murill polysaccharides in films improves full-thickness wound healing

• Published in: International journal of biological macromolecules Vol. 282; no. Pt 5; p. 137240
• Main Authors: Lima, Ana Beatriz Nogueira, Saraiva, Matheus Morais, Campelo, Matheus da Silva, Dias, Andre Tavares Freitas de Figueredo, Freires, Antônio Emanuel de Jesus, Ricardo, Nágila Maria Pontes Silva, Leitão, Renata Ferreira de Carvalho, Mattos, Adriano Lincoln Albuquerque, Sampaio, Tiago Lima, Ribeiro, Maria Elenir Nobre Pinho
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01-12-2024
• Subjects: Biomaterial; Flavonoid; Poly(vinyl) alcohol; Sodium alginate; Wound dressing; Wound dressing; Biomaterial; Poly(vinyl) alcohol; Sodium alginate; Flavonoid
• ISSN: 0141-8130; 1879-0003; 1879-0003
• DOI: 10.1016/j.ijbiomac.2024.137240

The present study aimed to associate troxerutin (TRX) with polysaccharides from Agaricus blazei Murill mushroom (PolyAb) in alginate/PVA films and evaluate their effect on wound healing. The physicochemical, mechanical, morphological, and water vapor barrier properties of the films were studied, and their biological potential was analyzed in vivo using the full-thickness wound healing model. The association between TRX and PolyAb present in the polymeric film contributed to increased thermal stability, mechanical resistance, and elasticity when compared to films without TRX, which indicated good miscibility of the excipients. In the in vivo tests, the TRX films promoted greater collagen deposition and repair of epidermis and dermis layers. The 0.25 % TRX film showed an increase in reduced glutathione levels, while the 1.0 % TRX film reduced lipid peroxidation and production of the pro-inflammatory cytokine IL-1β, demonstrating the anti-inflammatory and antioxidant effect of TRX films. Therefore, it is estimated that the film containing 1 % TRX can be used as biocurative for wound dressing applications.
•Troxerutin in films showed suitable mechanical properties for wound coverage.•Troxerutin exerted antioxidant and anti-inflammatory activity in healing process.•Troxerrutin decreases inflammation by modulating mast cell degranulation.•Troxerrutin stimulates nitric oxide and inhibits lipid peroxidation.