Biochemical and molecular-genetic properties of a cytochrome-c-deficient mutant ofKluyveromyces lactis

• Published in: Folia microbiologica Vol. 42; no. 4; pp. 319 - 323
• Main Authors: Hikkel, I., Gbelská, Y., Šubík, J., Lubec, G.
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Nature B.V 01-08-1997
• Subjects: Amino acids; Anomala; Bacteria; Bacteriology; Carbon sources; Cytochrome; Cytochrome c; Cytochromes; Deficient mutant; Glycerol; Homology; Microbiology; Nucleotide sequence
• ISSN: 0015-5632; 1874-9356
• DOI: 10.1007/BF02816942

We have isolated a respiration-deficient nuclear mutant of the yeastKluyveromyces lactis that exhibited diminished levels of all cytochromes and did not grow on glycerol and other nonfermentable carbon sources. The mutant namedcyc1 was transformed with aK. lactis genomic library and the DNA fragment conferring its wild-type properties was isolated and sequenced. The sequence of the isolated gene showed extensive homology with other eukaryotic cytochrome-c genes. The highest level of homology, based on the deduced amino acid sequences, was observed between the gene products ofK. lactis andHansenula anomala.