Zymographic analysis of lipolytical enzymes of subcellular Vibrio cholerae fractions of O1 and O139 serogroups

Zymographic analysis of lipolytical enzymes of subcellular Vibrio cholerae fractions of O1 and O139 serogroups

Zymographic analysis showed that all subcellular fractions of the serogroups possessed different degree of lipolytical activity. Maximal hydrolysis in the radial enzyme-diffusion reaction in agarose gel was observed in Tween-20, Tween-80, Triton Х-305 and Triton Х-405. Total hydrolase activity of ur...

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Bibliographic Details
Published in:Acta biomedica scientifica Vol. 1; no. 2; pp. 55 - 59
Main Authors: S. N. Kozlov, V. B. Nikolaev, E. Y. Markov, L. Y. Urbanovich, S. K. Mitkeeva
Format: Journal Article
Language:English
Russian
Published: Scientific Сentre for Family Health and Human Reproduction Problems 29-04-2016
Subjects:
lipolytical enzyme
subcellular fraction
substrate electrophoresis
vibrio cholerae
zymographic analysis
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Summary:Zymographic analysis showed that all subcellular fractions of the serogroups possessed different degree of lipolytical activity. Maximal hydrolysis in the radial enzyme-diffusion reaction in agarose gel was observed in Tween-20, Tween-80, Triton Х-305 and Triton Х-405. Total hydrolase activity of urea extracts in Tween-20, Tween-80, Triton Х-405, Span-85 excelled those in the preparations of outer membranes. Maximal lipolytical activity in regard to the Tweens was shown by the preparations of urea extracts prepared from the nontoxigenic strains of O1 serogroups, while in relation to the Tritons - preparations of urea extracts from the toxigenic strains (p < 0,05).
ISSN:2541-9420
2587-9596
DOI:10.12737/20616