P203 Usefulness of metalloproteinase-9 and tissue inhibitor of metalloproteinase-1 in clinical characterisation of children with newly diagnosed Crohn’s disease

• Published in: Journal of Crohn's and colitis Vol. 12; no. supplement_1; p. S201
• Main Authors: Daniluk, U, Daniluk, J, Guzinska-Ustymowicz, K, Pryczynicz, A, Sieczkowska-Golub, J, Kierkus, J, Lebensztejn, D
• Format: Journal Article
• Language: English
• Published: UK Oxford University Press 16-01-2018
• ISSN: 1873-9946; 1876-4479
• DOI: 10.1093/ecco-jcc/jjx180.330

Abstract
Background
Non-invasive markers for diagnosis and monitoring the Crohn’s disease outcome are urgently needed, especially in children. The aim of our study was to determine the usefulness of new non-invasive markers representing gut mucosal damage (Metalloproteinase-9, MMP-9) and remodelling (tissue inhibitor of metalloproteinase-1, TIMP-1) in clinical characterisation of children with newly diagnosed Crohn’s disease.
Methods
Serum and fecal MMP-9 and TIMP-1 concentrations were determined by ELISA in 26 children with CD and 21 controls. Based on Spearman’s analysis the correlations between each marker and serum CRP, ESR, CBC, albumin, endoscopic score (SES-CD), enteroclisis signs, and histology staining were tested. The differences in TIMP-1 and MMP-9 levels between children with various CD clinical manifestation were calculated using U Mann–Whitney analysis.
Results
CD children demonstrated significantly higher levels of serum, fecal and colon MMP-9 and TIMP-1 compared with controls (all p < 0.05). Fecal and serum TIMP-1 was higher in children up to 14 years old (respectively p = 0.015 and p = 0.035), however only fecal TIMP-1 was increased in patients with growth failure (p = 0.046) and in patients with L1–3 disease location (p = 0.028). Interestingly, serum TIMP-1 was higher in children with strictures found in colonoscopy (p = 0.002) and it was also correlated with platelet/albumin ratio (R = 0.524), CRP (R = 0.498), platelet count (R = 0.498) and SES-CD (R = 0.392). No differences in MMP-9 levels were found between CD groups, however fecal MMP-9 concentration was correlated with fecal calprotectin (R = 0.59) and the disease duration (R = −0.548). Based on MMP-9 and TIMP-1 histology score no correlations with tested parameters and differences between CD groups were found.
Conclusions
Serum and fecal TIMP-1 measurements may be useful not only as a diagnostic, but also as prognostic marker in newly diagnosed CD children. However, further studies to evaluate the usefulness of these markers are required for larger group of CD patients.