Purification of Human Monocytes on Microexudatecoated Surfaces
Abstract Human blood monocytes, but not other Ficoll-Hypaque cells, adhere to plastic surfaces from which confluent BHK cells have been removed. EDTA (3 mM) rapidly and completely removes monocytes, providing a simple technique for preparation of human monocytes in suspension. The method should be v...
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| Published in: | The Journal of immunology (1950) Vol. 120; no. 4; pp. 1372 - 1374 |
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| Main Authors: | , |
| Format: | Journal Article |
| Language: | English |
| Published: |
Am Assoc Immnol
01-04-1978
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| Online Access: | Get full text |
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| Summary: | Abstract
Human blood monocytes, but not other Ficoll-Hypaque cells, adhere to plastic surfaces from which confluent BHK cells have been removed. EDTA (3 mM) rapidly and completely removes monocytes, providing a simple technique for preparation of human monocytes in suspension. The method should be valuable for studies of monocyte function where monocyte monolayers are not suitable. |
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| ISSN: | 0022-1767 1550-6606 |
| DOI: | 10.4049/jimmunol.120.4.1372 |