99m Tc radiolabeling of polyethylenimine capped carbon dots for tumor targeting: synthesis, characterization and biodistribution

• Published in: International journal of radiation biology Vol. 97; no. 7; pp. 977 - 985
• Main Authors: Bayoumi, Noha A, Emam, Ahmed N
• Format: Journal Article
• Language: English
• Published: England 03-07-2021
• Subjects: Animals; Carbon - chemistry; Carbon - pharmacokinetics; Isotope Labeling; Mice; Polyethyleneimine - chemistry; Polyethyleneimine - pharmacokinetics; Quantum Dots - chemistry; Radiopharmaceuticals - chemistry; Radiopharmaceuticals - pharmacokinetics; Technetium - chemistry; Technetium - pharmacokinetics; Tissue Distribution; Tomography, Emission-Computed, Single-Photon; Tumor; Polyethylenimine; Carbon dots; Technetium-99m
• ISSN: 0955-3002; 1362-3095
• DOI: 10.1080/09553002.2021.1919781

Due to the favorable physicochemical properties and the biocompatibility, carbon dots (CDs) have gained a great attention as a tumor targeting agent. This study investigates polyethylenimine capped CDs (PEI capped CDs) as a prospective nanocarrier of technetium-99m (
Tc) for tumor targeting. Technetium-labeled CDs could be introduced as a promising candidate for single photon emission tomography (SPECT) imaging.
Polyethylenimine capped CDs were prepared by hydrothermal method using hyperbranched PEI and citric acid. For a purpose of comparison, citrate capped CDs were also prepared by microwave irradiation. Both types of CDs were characterized and radiolabeled with
Tc using sodium borohydride (NaBH
) as a reducing agent. Biodistribution and tumor targeting efficiency of the produced radiolabeled CDs have been studied in Earlich ascites tumor mice model.
Citrate capped CDs and PEI capped CDs have been synthesized successfully and characterized. High radiochemical yield of
Tc-citrate capped CDs
Tc-PEI capped CDs was obtained (97 ± 0.7 and 90 ± 0.2, respectively). Biodistribution studies of
Tc-labeled PEI capped CDs have shown a potential tumor uptake (10 ± 0.5% Radioactivity/gram tumor) with high target to non-target ratio (T/NT) around 7 at 1-h post injection.
Tc-citrate capped CDs have achieved a lower tumor uptake level (3.8 ± 0.3% Radioactivity/gram tumor 1 h post injection).
This study introduces PEI capped CDs as a promising nanocarrier of
Tc for efficient tumor targeting. Technetium-labeled PEI capped CDs could be utilized as a potential SPECT tumor imaging agent.