The expression of TP53 mRNA splice variants relates to progression of cancer in human colorectal cancer

• Published in: Pathology Vol. 46; p. S117
• Main Authors: Nozaka, Hiroyuki, Endo, Kentaro, Mayama, Akifumi, Takahashi, Kodai, Takami, Hideki, Kurose, Akira, Uesugi, Noriyuki, Ishida, Kazuyuki, Sugai, Tamotsu
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 2014
• ISSN: 0031-3025; 1465-3931
• DOI: 10.1097/01.PAT.0000454498.27191.3b

The carcinogenesis of colon and rectum is caused by accumulation of genetic alternation or mutation. Recent works have shown significant biomarkers that include loss of hetero-zygosity, gene mutations or gene methylation of genomic DNA. Furthermore, it is also reported that the functional analysis of mRNAs and miRNAs are significance for clarification of cancer progress. In this study, we examined the expression of TP53 mutation, TP53 mRNA splice variants, TP53 protein and TP53-related miRNA in human colorectal cancer, and evaluated significance as the clinicopathological biomarker.
Tumors were collected from 30 patients diagnosed with primary advanced colorectal cancer. Both genomic DNA and total RNA were extracted with TRIzol reagent, and TP53 mutation was analyzed by direct sequence method. TP53 mRNA splice variants were analyzed by RT-PCR, TP53-related miRNA were analyzed by qRT-PCR, and TP53 protein was detected by immunohisto-chemistry. Statistical analysis was carried out by SPSS21.
TP53 alfa showed significantly low expression in TP53 mutation cancer. TP53 beta showed significantly low expression in TP53 mutation cancer. TP53 gamma showed significantly high expression in TP53 mutation cancer, and it also showed low expression in pTMN stage I + II as compared to pTMN stage III + IV.