Efficient Regeneration of Carrot (Daucus carota L.) plants from cell suspension derived-callus

• Published in: Maǧallat Markaz buhūt al-taqniyyat al-ahyāʼiyyat (Online) Vol. 9; no. 1; pp. 58 - 63
• Main Authors: Mohammed, Amjad Abdul-Hadi Mohammed, Al-Mallah, Mozahim Kassim Al-Mallah
• Format: Journal Article
• Language: English
• Published: Al-Nahrain University/ Biotechnology Research Center 01-01-2015
• Subjects: carrot plants, Multiple Drop Array, cell suspension callus, beta-carotene, anthocyanin
• ISSN: 1815-1140; 2708-1370
• DOI: 10.24126/jobrc.2015.9.1.408

This study succeeded in establishment carrot cell suspension cultures from stem callus in liquidMS medium containing 1.0 mg L-1of each NAA and BA. Its density approach 3.4 ×105cell ml-1atthe third day of culture. These cells ,continued, when embedded in agar drop using MultipleDrop Array (MDA) technique, in division and forming cellular colonies which producingnumerous callus primordia that developed to callus cultures. When transferred to the soliddifferentiation medium (MS+ 1.0 mg L-1 NAA+ 1.5 mg L-1 BA), two hundreds and seventy-threeshoots produced. They readily rooted in agar-solidified MSO medium after three weeks andadapted in conditions of culture room. They were not transferred to field due to the unfavorableenvironmental conditions at that time. Cell suspension-derived callus tissues contained 82.62 μganthocyanin gm-1 of callus fresh weight, and 0.555 mg beta-carotene 100 gm-1of callus freshweight in the third period of extraction compared with their quantity calculated in stems calluswhich recorded 52.4 μg gm-1and 1.988 mg gm-1respectively.