Influence of Tempetature on Viability and Deveplopment of Heterakis gallinarum (Nematoda, Heterakidae) Eggs

• Published in: Zoodiversity (Online) Vol. 58; no. 5; pp. 435 - 444
• Main Authors: Yevstafieva, V., Omelchenko, O., Melnychuk, V., Nagorna, L., Petrenko, M., Shaferivskyi, B., Kravchenko, S., Suprunenko, K., Karysheva, L., Sheiko, S., Burda, T., Syzonenko, N., Savenkova, O., Liulka, V.
• Format: Journal Article
• Language: English
• Published: Publishing House "Akademperiodyka" 01-08-2024
• Subjects: embryogenesis; heterakis gallinarum; histomoniasis; nematode eggs; survival rate; temperature regime
• ISSN: 2707-725X; 2707-7268
• DOI: 10.15407/zoo2024.05.435

Pinworms of the genus Heterakis Dujardin, 1845 parasitize a wide range of hosts, including a number of species of domestic and wild birds. These nematodes are ecologically adapted to the climatic conditions of many geographical zones, which contributes to their widespread distribution. The aim of the work was to investigate the influence of different temperature regimes at laboratory conditions on the growth, development and viability of eggs isolated from the gonads of female nematodes Heterakis gallinarum Schrank, 1788. The conducted studies established that, depending on the cultivation temperature, nematode eggs developed from 6 to 56 days, and their survival rate was from 67.7 to 83.7 %. The temperature of 25 °С was the most favourable for the development of H. gallinarum eggs, the development lasted 12 days and the highest number of eggs with motile infective larvae was formed (83.7 %). It was found that the period of egg development in cultures gradually decreased with increasing temperature: it was 56 days at 15 °C, 18 days at 20 °C, 12 days at 25 °C and 6 days at 30 °C. The survival rate of H. gallinarum eggs gradually increased with increasing temperature in regimes of 15 °С, 20 °С and 25 °С to 67.7 %, 80.7 % and 83.7 %, respectively. At a temperature of 30 °C, egg survival decreased slightly to 78.7 %. It was found that the development of H. gallinarum eggs from the zygote stage to the formation of motile infective larvae, depending on the temperature regime of cultivation, is accompanied by a decrease in egg length by 4.9–5.4 %, an increase in egg width by 4.1–7.6 % and a thinning of the shell by 9.5–28.6 %. Under different temperature regimes, the morphometric parameters of eggs during their development differ in terms of their length, width and shell thickness.