Expression of tobacco cDNA encoding phytochelatin synthase promotes tolerance to and accumulation of Cd and As inSaccharomyces cerevisiae

Expression of tobacco cDNA encoding phytochelatin synthase promotes tolerance to and accumulation of Cd and As inSaccharomyces cerevisiae

The first tobacco cDNA encoding phytochelatin synthase (NtPCS1) has been cloned by complementing the YCF1 (vacuolar ABC type transporter)-depleting yeast mutant DTY167 with an expression library fromNicotiana tabacum. When NtPCSI was over-expressed in DTY165 (WT) and DTY167 (mutant), tolerance to an...

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Bibliographic Details
Published in:Journal of plant biology = Singmul Hakhoe chi Vol. 48; no. 4; pp. 440 - 447
Main Authors: Kim, Young Jin, Chang, Kwang Seok, Lee, Mi Ran, Kim, Jong Hoon, Lee, Chang Eun, Jeon, Yoon Joo, Choi, Jong Soon, Shin, Hyoung Sun, Hwang, Seongbin
Format: Journal Article
Language:English
Published: Heidelberg Springer Nature B.V 01-12-2005
Subjects:
Accumulation
Arsenic
Cadmium
Coding
Heavy metals
Mutants
Phytochelatin synthase
Tobacco
Yeasts
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Summary:The first tobacco cDNA encoding phytochelatin synthase (NtPCS1) has been cloned by complementing the YCF1 (vacuolar ABC type transporter)-depleting yeast mutant DTY167 with an expression library fromNicotiana tabacum. When NtPCSI was over-expressed in DTY165 (WT) and DTY167 (mutant), tolerance to and the accumulation of cadmium (Cd) were enhanced. Interestingly, its expression promoted these responses as well to arsenic (As), but only in DTY167. We conclude thatNtPCS1 plays a role in tolerance to and the accumulation of both toxic metals inSaccharomyces cerevisiae.
ISSN:1226-9239
1867-0725
DOI:10.1007/BF03030586