Replication study of 34 common SNP s associated with prostate cancer in the Romanian population

• Published in: Journal of cellular and molecular medicine Vol. 20; no. 4; pp. 594 - 600
• Main Authors: Jinga, Viorel, Csiki, Irma Eva, Manolescu, Andrei, Iordache, Paul, Mates, Ioan Nicolae, Radavoi, Daniel, Rascu, Stefan, Badescu, Daniel, Badea, Paula, Mates, Dana
• Format: Journal Article
• Language: English
• Published: Chichester John Wiley & Sons, Inc 01-04-2016
• Subjects: Alleles; Biopsy; Chromosome 4; Chromosome 6; Deoxyribonucleic acid; DNA; Family medical history; Genetic analysis; Genetic diversity; Genetic research; Genetic variance; Genomes; Phenotypes; Population; Prostate; Prostate cancer; Replication; Single-nucleotide polymorphism; Statistical analysis; Studies; Tumors; Ultrasonic imaging; Urology; Romania; United States > US; Massachusetts; Iceland
• ISSN: 1582-1838; 1582-4934
• DOI: 10.1111/jcmm.12729

Prostate cancer is the third‐most common form of cancer in men in Romania. The Romanian unscreened population represents a good sample to study common genetic risk variants. However, a comprehensive analysis has not been conducted yet. Here, we report our replication efforts in a Romanian population of 979 cases and 1027 controls, for potential association of 34 literature‐reported single nucleotide polymorphisms (
SNP
s) with prostate cancer. We also examined whether any
SNP
was differentially associated with tumour grade or stage at diagnosis, with disease aggressiveness, and with the levels of
PSA
(prostate specific antigen). In the allelic analysis, we replicated the previously reported risk for 19 loci on 4q24, 6q25.3, 7p15.2, 8q24.21, 10q11.23, 10q26.13, 11p15.5, 11q13.2, 11q13.3. Statistically significant associations were replicated for other six
SNP
s only with a particular disease phenotype: low‐grade tumour and low
PSA
levels (rs1512268), high
PSA
levels (rs401681 and rs11649743), less aggressive cancers (rs1465618, rs721048, rs17021918). The strongest association of our tested
SNP
's with
PSA
in controls was for rs2735839, with 29% increase for each copy of the major allele G, consistent with previous results. Our results suggest that rs4962416, previously associated only with prostate cancer, is also associated with
PSA
levels, with 12% increase for each copy of the minor allele C. The study enabled the replication of the effect for the majority of previously reported genetic variants in a set of clinically relevant prostate cancers. This is the first replication study on these loci, known to associate with prostate cancer, in a Romanian population.